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Worksheets

Aruzhan Abdrakhman

Total questions: 7

Worksheet time: 70secs

Name
Class
Date
1.

to slow down the degradative action (breaking down tissue) of hydrolytic enzymes on organelles

a)

Why must the isolation medium be buffered?

b)

Why must the isolation medium be isotonic?

c)

What are the three steps of Cell Fractionation?

d)

Why must the isolation medium be cold?

2.

Grinding up cells may release H+ or H-

a)

Why is acidity/alkalinity affected when you homogenise a tissue?

b)

What are the three criteria that the isolation medium must be?

c)

What is filtration?

d)

Why do we do it to the sample?What cells are the toughest to homogenise & why?

3.

To lyse the cells → Why do we homogenise tissue?

a)

Верно

b)

Неверно

4.

Plant cells because of their tough cellulose wall → What does the mass of the organelle determine?

a)

Верно

b)

Неверно

5.

To lyse the cells

a)

Why do we homogenise tissue?

b)

What do we use to fractionise the cell?

c)

What does homogenise mean?

d)

What do you grind up with the tissue?

6.

Grind up → What does homogenise mean?

a)

верно

b)

неверно

7.

salty water

-sugary water

a)

Give example of an isotonic solution

b)

What do you grind up with the tissue?

c)

Why do we homogenise tissue?

d)

What do we use to fractionise the cell?