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WorksheetsRevision Recombinant DNA Technology
Total questions: 12
Worksheet time: 6mins
Restriction enzymes are
DNA unwinding enzymes
DNA joining enzymes
DNA cleaving enzymes
DNA synthesis enzymes
The enzyme used to join the DNA fragments
Topoisomerase
DNA ligase
DNA polymerase
Reverse transcriptase
Which one or more of these can be used as a vector?
i. Bacterium ii. Plasmid iii. E. coli iv. Bacteriophage
i & ii
ii & iii
i & iii
ii & iv
An organism that has had its genes manipulated by genetic engineering is considered as ___.
transgenic
crossbred
transmutant
culitvars
Which is NOT an advantage of modern genetic manipulation?
It allows scientists to manipulate genetic features by breeding animals with desired traits.
It allows scientists to research for possible cures to genetic diseases.
It allows scientists to have direct influence over an organism's genes.
It allows scientists to introduce novel genes into an organism.
Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
restriction endonuclease cleaving gene of interest and plasmid.
production of recombinant DNA using DNA ligase and a mixture of cut gene of interest and plasmid.
transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin.
probing blots of bacteria clones with radioactive DNA complementary to the desired gene.
Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its uses?
restriction endonuclease - production of DNA fragments for gene cloning.
DNA ligase - enzyme that cuts DNA, creating sticky ends.
reverse transcriptase - production of cDNA from mRNA.
Taq polymerase - copies DNA sequences in the polymerase chain reaction.
5'GAATTC3' is a restriction site widely used by restriction enzyme which produces sticky ends. The enzyme is
BamHI
HindIII
EcoRI
SmaI
Which of the following is correct sequence of process in recombinant DNA technology?
- Isolation of target DNA & vector
- Introducing recombinant DNA into host cell
- Fragmentation of target DNA & vector by restriction endonuclease
- Ligation of DNA fragment into vector
- Culturing in host cell to get desired products.
1,2,3,4,5
1,3,4,2,5
1,3,4,2,5
1,4,3,2,5
Which of the following is used as selectable marker?
Ampicillin resistance gene
Plasmid resistance gene
Salmonella resistance gene
Penicillin resistance gene
Which of the following is NOT belong to host cell
Able to accept recombinant DNA
Able to accept accept target DNA
Able to maintain structure of recombinat DNA
Able to express the gene of interest
Which of the following is correct sequence of process in production of cDNA?
- Reverse transcriptase used to synthesis single stranded cDNA complementary to mRNA
- Remove mRNA
- Extract mRNA from cell
- Use DNA polymerase to synthesis second stranded cDNA
1,2,3,4
3,4,2,1
3,1,2,4
2,3,1,4
