WorksheetsELISA
Total questions: 20
Worksheet time: 10mins
When a positive + result occurs in an ELISA test, then one could conclude that...
a + result always means illness
the antibody is present
it is a false + and no illness
an enzyme is present
bind sample to well
add primary Ab and wash
add secondary Ab/enzyme and wash
add substrate
Why is there an enzyme attached to the secondary antibody?
They link to the proteins used
The catalyze reactions
They provide a colormetirc indication of antigen exposure
They are used to detect DNA
A sample of spinal fluid is taken from a college student suspected to have contracted spinal meningitis.Which diagnostic test will confirm the diagnosis fastest?
Culturing the spinal fluid and testing for bacterial colony growth in a Petri dish.
An ELISA Assay to determine the presence of menigitis antigens.
DNA sequencing
Protein Chromatography
The role of an antibody is to
Mark antigens for destruction
Provide a weapon that will stick to and invade surrounding cells
To attack your own body
Promote and attract potential pathogens
Which of the following cannot be determined by an ELISA test?
Concentration of antigens
Presence of disease
Genetic sequence of a pathogen
Order of infection
After adding the secondary antibody and enzyme complex to the ELISA, what is added next (after washing?)
substrate
primary antibody
enzyme #2
antigen
A doctor orders an ELISA test for two patients. The results show a color change from clear to blue. Patient A results are a dark blue while the results for patient B are a light blue. What is the most likely diagnosis?
Patient A is more infected
Patient B is more infected
The patients are infected equally
Neither patient is infected
How the ELISA used in class both a quantitative and a qualitative test?
ELISA yields a plus or minus sign for presence of antigen and a number for amount present.
ELISA yields a color change to indicate the presence of the antigen and a generates a number for the amount of antigen present
ELISA yields a color change for presence of antigen and the intensity of the color indicates the amount present
There is a computer printout with the results of the test and the amount present
What is a primary antibody?
First step in ELISA that binds to the antigen
The first antibody
the bacteria
a chicken antibody
The A in ELISA stands for
allele
antigen
assay
antibody
What does the secondary antibody bind to?
antigens
the microplate
the primary antibody
the virus of interest
