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REVISION CHAPTER 7 & 8

Total questions: 20

Worksheet time: 12mins

Name
Class
Date
1.
What kind of mutation is the result of an insertion?
a)
Silent Mutation
b)
Missense mutation
c)
Nonsense mutation
d)
Frameshift mutation
2.
A base pair substitution can result in all of the following except __________.
a)
Missense mutation
b)
Frameshift Mutation
c)
Silent Mutation
d)
Nonsense mutation
3.
An example of a mutation where a piece of chromosome breaks off and attaches to another chromosome is called ___.
a)
trisomy
b)
translocation
c)
inversion
d)
deletion
4.

Notice the abnormal 23rd chromosome. This individual suffers from which chromosomal genetic disorder?

a)

Klinefelter Syndrome

b)

Down Syndrome

c)

Cri-du-Chat Syndrome

d)

Andrew Syndrome

5.

Chromosomal number alterations refer to __________.

a)

Changes in structure of chromosome

b)

Changes in sequence of bases in DNA

c)

Changes in numbers of chromosomes

d)

Changes in numbers of organisms

6.

A person with Cri-du-Chat Syndrome is

a)

missing chromosome # 5

b)

trisomy 13

c)

trisomy 21

d)

missing part of chromosome # 5

7.

Another name for trisomy 21 is

a)

Patau Syndrome

b)

Down syndrome

c)

Edward syndrome

d)

Turner syndrome

8.

Klinefelter Syndrome is caused by the presence of

a)

an extra Y-chromosome (XYY)

b)

an extra X-chromosome (XXY)

c)

a missing X-chromosome ( _X)

d)

trisomy 21

9.
What process occurs during meiosis that can result in chromosomal disorders?
a)
crossing over
b)
non-disjunction
c)
anaphase I
d)
prophase II
10.

Plant A (2n=10) mates with plant B (2n=12) produce a sterile hybrid which evolved to a fertile hybrid. What is the chromosome number of a fertile hybrid?

a)

33

b)

44

c)

11

d)

22

11.
Which of the following is a circular DNA from bacteria that can hold a foreign gene?
a)
plasmid
b)
restriction enzyme
c)
ligase
d)
protein expression
12.
Which of the following is a protein that acts as DNA scissors?
a)
plasmid
b)
restriction enzyme
c)
ligase
d)
protein expression
13.
What is the enzymatic function of restriction enzymes?
a)
To add new nucleotides to the growing strand of DNA.
b)
To join nucleotides during replication.
c)
To join nucleotides during transcription.
d)
To cleave nucleic acids at specific sites.
14.

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

a)

amplification

b)

insertion

c)

transformation

d)

screening

15.

The cell that able to receive recombinant DNA for cloning purpose known as

a)

vector

b)

target DNA

c)

plasmid

d)

host cell

16.

To produce desired protein _____________ should be taken from any source like human, animal and plant.

a)

recombinant DNA

b)

Target DNA

c)

plasmid

d)

ligase

17.

Arrange a correct sequence of process in production of cDNA.


I. Reverse transcriptase used to synthesis single stranded cDNA complementary to mRNA

II. Remove mRNA

III. Extract mRNA from cell

IV. Use DNA polymerase to synthesis second stranded cDNA

a)

I, II, III, IV

b)

III, IV, II, 1

c)

III, I, II, IV

d)

II, III, I, IV

18.

Which of the characteristic is NOT belong to host cell

a)

Able to accept recombinant DNA

b)

Able to accept target DNA

c)

Able to maintain structure of recombinat DNA

d)

Able to express the gene of interest

19.

Which gene is used as selectable marker in a plasmid?

a)

Ampicillin resistance gene

b)

Plasmid resistance gene

c)

Salmonella resistance gene

d)

Penicillin resistance gene

20.

Which of the following is a correct sequence of process in recombinant DNA technology?


I. Isolation of target DNA & vector

II. Introducing recombinant DNA into host cell

III. Fragmentation of target DNA & vector by restriction endonuclease

IV. Ligation of DNA fragment into vector Culturing in host cell to get desired products.

a)

I, II, III, IV

b)

I, III, IV, II

c)

I, II, IV, III

d)

I, IV, II, III