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BIP biochemistry and organic journal

Total questions: 10

Worksheet time: 21mins

Name
Class
Date
1.

A bioenhancer is an agent which increases bioavailability and bioefficacy of a particular drug with which it is combined; it does not have any typical pharmacological activity of its own at the dose used. It can be inferred that bioenhancer …

a)

have own particular pharmacological activity

b)

do not have own particular pharmacological activity

c)

can increase bioavailability itself

d)

can increase bioefficacy itself

2.

Extraction by using various solvents is another method in which piperine is extracted by using solvents like ethanol 16,dichloromethane 17 and glacial acetic acid 18. In this method extraction of the fruit powder with glacial acetic acid is done. The piperine extract obtained is further partitioned into chloroform. Purification of piperine is done by column chromatography along with toluene and ethyl acetate (7:3) 19 as a solvent can be used. The folowing is solvent for piperine extraction except ...

a)

ethanol

b)

dichloromethane

c)

acetic acid

d)

ethyl acetat

3.
The dried prepared sample was subjected to column chromatography (CC), using a 38 X 4.5 cm glass column filled with silica gell 60 (mesh size: 60–120#) in toluene: ethyl acetate(7:3). Based on that sentence, we know that stationery phase of column chromatography was made of …
a)
toluene
b)
ehthyl acetate
c)
silica heterogen
d)
silica homogen
4.
Piperine was isolated from black pepper by using three different solvents ethanol, dichloromethane,glacial acetic acid which gave piperine yield 3.2%,5% and 4.6% respectively. The yield of piperine was found out to be more in dichloromethane extract but the final product obtained was not crystalline in nature. The product obtained from the other method where piperine was extracted with glacial acetic acid produced clear needle shaped piperine crystals. Based on the text, the best solvent to get chrystalline piperine is ...
a)
ethanol
b)
dichloromethane
c)
glacial acetat
d)
other solvent
5.
Because of depletion of world petroleum reserves and increasing environmental concerns, biodiesel, a renewable and biodegradable fuel, is an ideal resource to replace fossil fuel. The subject of this sentence is ....
a)
depletion
b)
petroleum and increasing environmental concern
c)
biodiesel
d)
renewable and biodegradable fue
6.
The temperature in hot springs is usually over the limit of eukaryotic life (near to 60 C), which limits the microbial life to Bacteria and Archaea (and their viruses). Thermostable enzymes from thermophilic microorganisms are important biocatalysts for industrial and biotechnological purposes, given that they can work at high temperatures in which mesophilic enzymes would bedenatured. Based on the text, the microorganisme that can be survive in temperature over 60oC is ...
a)
bacteria and archaea
b)
thermopilic microorgnism
c)
biocatalyst
d)
mesophylic enzim
7.
About 20 Mb of mixed microbial DNA was extracted directly from microbes in the Eryuan Niujie hot spring of Yunnan Province according to Rondon's method. Briefly, a cell pellet embedded in low-melting-point agarose was immersed in a lysis solution with Hind III for 20 min at 37 C. The word "immersed" means ...
a)
to be drawn into
b)
to be thrown into
c)
to be absorbed into
d)
to burried into
8.
The effect of temperatures ranging transesterification activity for biodiesel production is shown in Fig. 4. Results showed (Fig. 5) that there was a rise yield from 25 to 55 C on lip-1 in the biodiesel yield catalyzed by AOB-sole-lip-1 when the temperature was gradually increased from 25 to 45 C and the maximal biodiesel yield was obtained at 45 C, but further increments in temperature to 55 C caused a decrease in the biodiesel. The following appropriate answer that can be concluded from the text is ...
a)
the maximum condition temperature is 45oC
b)
the maximum condition temperature is 55oC
c)
the maximum condition temperature is 25oC
d)
It increases from 25-55oC
9.
Clones which were randomly selected were inoculated into LB medium from the BAC library(containing 12.5 mg/mL chloramphenicol) at 37 C overnight. This text will be found in the section…
a)
introduction
b)
method
c)
result and discussion
d)
conclusion
10.

Lipase-mediated enzymatic transesterification can facilitate biodiesel production. Because of their site selectivity, chemical selectivity, substrate selectivity, and

lower toxicity, lipases are promising enzymes for use in detergents, food processing, organic cosmetics, and the pharmaceutical industry. Currently, many microorganisms (including bacteria, yeasts and fungi) are considered as potential extracellular lipase producing strains. Although lipases exist widely in higher plants

and animals, microbial lipases are getting more attention due to their abundant gene resources, high stability, and broad substrate specificity. We can observe that lipase was preferred from microbial lipasses than higher plants due to following criteria except ...

a)

site selectivity

b)

chemical selectivity

c)

high stability

d)

higher than higher plant