WorksheetsPCR
Total questions: 10
Worksheet time: 5mins
The PCR technique was developed by
Karry Mullis
Kohler
Milstein
Altman
PCR is a
DNA Sequencing technique
DNA Amplification technique
DNA Degradation technique
All of these
Which of the following statements are true regarding PCR
Automated PCR machines are called thermal cyclers
A thermostable DNA Polymerase is required
Billion copies of desired DNA can be synthesized from microgram quantities of DNA
All of these
Thermus aquaticus is the source of
Primase enzyme
Taq Polymerase
Vent Polymerase
Both 2 and 3
All of the following are thermostable polymerases except
Taq Polymerase
Vent Polymerase
DNA Polymerase III
pfu Polymerase
the basic requirements of PCR reaction include
DNA segment to be amplified
Two oligonucleotide primers
a heat stable DNA polymerase
all of these
The first step in PCR is
Primer annealing
Denaturation
Primer extension
None of these
Denaturation involves
heating between 40-60 C
heating between 90-98 C
heating between 70-72 C
None of these
Reverse transcription PCR uses
mRNA as a template to form
RNA as a template to form a DNA
DNA as a template to form ssDNA
all of these
Which of the following is true regarding PCR
Denaturation involves heating at 90-98 degree C
Annealing involves binding of primer between 40-60 degree C
Primer extension occurs at 72 degree C
All of these
