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GCMB-604

Total questions: 20

Worksheet time: 20mins

Name
Class
Date
1.

Which of the following equation shows DNA renaturation reaction?

a)

Sec 60

b)

Cot1/2

c)

Tan 30

d)

Cot 40

2.

What is the relationship among DNA, a gene, and a chromosome?

a)

A chromosome contains hundreds of genes, which are composed of DNA.

b)

A chromosome contains hundreds of genes, which are composed of protein.

c)

A gene contains hundreds of chromosomes, which are composed of protein.

d)

A gene is composed of DNA, but there is no relationship to a chromosome

3.

The DNA binding proteins bind at the _______________

a)

Minor groove

b)

Major groove

c)

Phosphate molecules

d)

Pentose sugars

4.

During DNA denaturation, which of the following occurs?

a)

Unwinding of DNA double strand

b)

Absorbance of UV rays

c)

Decrease in hydrophobic interactions of base stacking

d)

All of the mentioned

5.

Euchromatin chromosomes having characteristic feature contain

a)

stains lightly

b)

is partially condensed

c)

genetically active chromatin with genes

d)

all of the above

6.

Which of the following statement is NOT true in eukaryotic system?

a)

The genetic material lies within the cytoplasm of cell

b)

The 22 pairs of chromosomes are known as autosomes

c)

Gene contains code that makes specific type of protein

d)

DNA contains 2 strands

7.

The 5’ and 3’ number are related to the

a)

Length of the DNA strand

b)

Carbon no. in the sugar

c)

The Number of Phosphate

d)

The base pair rule.

8.

RNA is generally single stranded molecule. Which statement is incorrect regarding this molecule?

a)

RNA contain uracil

b)

RNA can only be found inside the nucleus

c)

RNA is a single stranded molecule

d)

RNA contains genetic information

9.

Which molecules are part of RNA and DNA both?

a)

Uracil

b)

Thymine

c)

Nucleotides

d)

Deoxyribose sugar

10.

How does genome complexity of denatured DNA measure?

a)

Giemsa staining

b)

Reverse chromatography

c)

Denaturation kinetics

d)

Renaturation kinetics

11.

All of the following are true about nucleotide excision repair except.

a)

Removal of the damage base occurs on only one strand of the DNA

b)

It involves the activity of an excision nuclease, which is endonuclease

c)

It requires a polymerase and ligase

d)

Only the damaged nucleotides are removed.

12.

The sigma factor subunit of prokaryotic RNA polymerase

a)

Is part of the core coenzyme

b)

Bind the antibiotic rifampicin

c)

Must be present for transcription to occur.

d)

Specially recognizes promoter sites

13.

What is the main damaging effect of UV radiation on DNA

a)

Depurination

b)

Formation of Thymine Dimers

c)

Single strand breaks

d)

Dehydrations.

14.

What is the only common methylation in the DNA of eukaryotics.

a)

Adenosine in cPA dinucleotide

b)

Guanosine in APCPA trinucleotide

c)

Cytosine in CPG dinucleotide

d)

None of the above

15.

Which of the following enzymes are used to join segment of DNA

a)

DNA ligase

b)

DNA polymerase

c)

Primase

d)

Endonuclease

16.

The first protein complex to bind the TATA box is

a)

Transcription factor IIB

b)

Transcription factor IIA

c)

Transcription factor IID

d)

All of the above

17.

In eukaryotes there are three different RNA polymerase.The RNA polymerase responsible for transcription of mRNA is

a)

RNA polymerase I

b)

RNA polymerase II

c)

RNA polymerase III

d)

All of the Above

18.

Name the sequence of RNA, which is recognized by a small subunit of the ribosome.

a)

Rho utilization site

b)

Downstream sequence

c)

Upstream sequence

d)

Shine Dalgarno sequence

19.

Before RNA polymerase can initiate transcription, this protein must bind to it, creating the RNA polymerase holoenzyme and allowing for the initiation of transcription.

a)

Topoisomerase

b)

Sigma factor

c)

Helicase

d)

Activator protein

20.

Each replication Bubbles contain.

a)

4 Replication forks

b)

2 Replication forks

c)

8 replication Forks

d)

None of the replication forks.