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WorksheetsMidterm Biotech 2
Total questions: 50
Worksheet time: 25mins
Determines the amount and length of activity for a given protein in a test organism
activity assay
PK/PD assay
toxicology assay
stability assay
When multiple samples of known concentrations are measured and graphed so that the concentration of an unknown can be determined is a
standard curve
abnormal curve
ELISA
isoelectric point
Prism-like structure that separates light waves
grating
visible light spectrum
nanometer
a.u.
What organism can naturally produce amylase?
cat
algae
fungi
dog
Which of the following is an indicator used in protein concentration determinations?
ELISA indicator
BPA solution
Bradford reagent
BCIP
What is amylase?
an enzyme
a sugar
a polysaccharide
a starch
Phase of the product pipeline where large volume production of the drug occurs
research and development
manufacturing
clinical trials
Phase of the product pipeline where toxicity and efficacy studies are performed on mice
research and development
manufacturing
clinical trials
Unit of light absorbance
mg/ml
absorbance
a.u.
transmittance
A mass spectrometer may be used for all of the following except?
determining amino acid composition
determining the molecular mass of a compound
determining the chemical analysis of paints dyes, poisons
determining the genetic code of a DNA sample
Which instrument is used for determining the concentration of melanin pigment
UV spec
VIS spec
The pH at which a protein's charge becomes neutral and the protein precipitates out of solution.
amino acid
isoelectric point
density
acid point
What type of experiment is used to test experimental procedures in a limited basis before a full experimental study?
concentration assay
pilot study
western blot
gel electrophoresis
All unauthorized experiments are prohibited.
True
False
A tech sees a 55 kDA band on a PAGE gel and suspects it is amylase. How can she confirm it is?
Eastern blot
Northern blot
Southern blot
Western blot
If the concentration of lactase needed to be measured using Bradford reagent, which instrument would be used?
thermocycler
spectrophotometer
fermenter
capillary detector
Molecules, produced by bacteria or fungus, which inhibit bacterial growth
formulation
transfection
assay
antibiotic
A way to determine how long the pharmaceutical is effective and how it should be stored
activity assay
PK/PD assay
toxicology assay
stability assay
Approved eye protection devices (such as goggles) are worn in the laboratory
to avoid eye strain
to improve your vision
only if you don't have corrective glasses
any time chemicals, heat or glassware are used
Light with wavelengths between 200-350 nm
mg/ml
grating
spectrophotometer
UV light
The concentration of protein in samples can be estimated by comparing to a
bell curve
standard curve
average curve
milligram curve
A spectrophotometer must be calibrated before every use.
True
False
Which indicator was used to detect amylase because it turns from brown to blue in the presence of starch?
acid
pipet
iodine
casein
Herbal therapies have been used in some cultures for hundreds of years, but caution should be taken for all of these reasons, EXCEPT
they're not subject to FDA approval
adverse effect may occur from the wrong dose
they taste better
certain combos could be harmful
ELISAs are frequently used to assay for proteins during the manufacturing because
they are very general
they are very specific
only occasional samples need to be taken
a simple indicator test is sufficient
A test for determining the presence or a particular characteristic of a protein
formulation
transfection
assay
antibiotic
Helps researchers measure the amount of protein present
concentration assay
PK/PD assay
toxicology assay
stability assay
Flammable materials, like alcohol, should never be dispensed or used near
an open door
an open flame
another student
a sink
The absorbance of known concentrations of protein on a standard curve allows the determination of?
protein size
protein shape
protein concentration
protein activity
Which instrument can recognize the molecular weight of a compound?
UV spec
VIS spec
mass spec
IR spec
An instrument that shines light and measures the amount of light transmitted through a sample
mg/ml
grating
spectrophotometer
transmittance
Unit of protein concentration
mg/ml
grating
spectrophotometer
transmittance
Which instrument is used for checking for the presence of an unknown colorless protein
UV spec
VIS spec
Instead of multi-tasking, humans generally take turns completing tasks, which is called
multi-tasking
procrastination
vibe checking
switch tasking
Horseplay or practical jokes in the laboratory are
okay if you are working alone
not dangerous
okay
always against the rules
What is the most convenient way to determine the absorbance spectrum of a single sample?
UV/Vis spec
balance
pH paper
mass spec
The wavelengths of light that the human eye can see
mg/ml
visible light spectrum
spectrophotometer
transmittance
Used to determine what are safe and unsafe levels of a given drug
concentration assay
ELISA
toxicology assay
stability assay
If a lab experiment is not completed, you should
make up some results
come in during lunch and finish while eating lunch
sneak in after school and work alone
discuss the issue with your instructor
Never leave a lit burner unattended.
True
False
Chipped or cracked glassware is okay to use.
True
False
If a laboratory fire erupts, immediately
notify your instructor
run for the fire extinguisher
throw water on the fire
open the windows
Recombinant DNA
is used to produce 100's of other DNA products
can be found in fungus cells
is produced by pasting DNA from 2 sources together
is larger than a typical chromosome
Phase of the product pipeline where safety and efficacy studies on human subjects are monitored by the FDA
research and development
manufacturing
clinical trials
The following footwear is best in the laboratory.
shoes appropriate for the weather
open-toed shoes
closed-toed shoes
sandals
If you do not understand a direction or part of a lab procedure, you should
skip it and go on to the next part.
ask the instructor before proceeding
try several methods until something works
figure it out as you do the lab
The amount of transmittance is related to the amount of absorbance by?
T/A equation
transmittance assays
absorbance indication
Beer's law
Amount of light that passes through a sample
UV light
visible light spectrum
transmittance
a.u.
The wavelength at which a sample absorbs the most light
absorbance
Lamba(max)
nanometer
a.u.
You are heating a piece of glass and now want to pick it up. You should
pour cold water on it
use tongs
pick up the end that looks cooler
use a rag or paper towels
