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STC assessment 4

Total questions: 20

Worksheet time: 15mins

Name
Class
Date
1.

Adaptation phase in CRISPR-Cas operates by

a)

Blocking entry of viral nucleic acid

b)

Activating Restriction Enzymes

c)

Integrating a short piece of viral DNA in CRISPR locus

d)

none of the above

2.

For Genome editing a double stranded cut at the target DNA is required

a)

True

b)

False

3.

CRISPR genome editing works both in eukaryotes and prokaryotes

a)

True

b)

False

4.

The nuclease function of Cas9 has to be removed for gene silencing

a)

True

b)

False

5.

Off-target effects are not a concern for CRISPR mediated genome editing

a)

True

b)

False

6.

The binding of CRISPR RNA alone with the incoming viral DNA is sufficient to protect the bacterial cell

a)

True

b)

False

7.

In the context of a data, information and noise are relatively defined terms with respect to the researcher’s perspective. The perspectives change and so do the label.

a)

True

b)

False

8.

Would all modified (Crossover in GA) products be novel?

a)

Yes

b)

No

c)

May be

d)

66% of them will be novel

9.

Started with phrases of one note and three-consecutive-notes the string SagaRe in Indian classical music could be obtained by ________

a)

Mutation

b)

Crossover

c)

Either of them

d)

None of them

10.

AI models could possibly be employed for ________ out of the following 6 applications

a. Identification b. Classification c. Prediction

d. Error correction e. Validation f. Missing data recovery

a)

3

b)

5

c)

6

d)

None of these

11.

Smart devices are the products of Technology _______?

a)

2.0

b)

3.0

c)

4.0

d)

(ii) and (iii)

12.

16S rDNA is the gene of choice for deciphering the composition of microbes in a sample. Why?

a)

conserved at amino acid level

b)

highly divergent

c)

alternating conserved and variable regions

d)

not found in eukaryotes

13.

If the amplicon size is 350 bases and the two paired-end reads from the same amplicon are 200 bases long, the length of the overlap between the paired end reads are

a)

150

b)

100

c)

75

d)

50

14.

N50 is

a)

measure of gene expression

b)

quality of assembly

c)

concentration of DNA

d)

model of sequencing machine

15.

How will you detect mutation that is a potential cause for a rare familial recessive disorder

a)

look for mutation in one arm

b)

look for homozygous alternate allele in both arms

c)

look for heterozygous alternate allele in both arms

d)

none of the above

16.

Bisulfite treatment of DNA results in following changes

a)

all T become C

b)

all methylated C become T

c)

all unmethylated C become T

d)

all A becomes C

17.

Which of these molecular biology platforms has the best resolution?

a)

Karyotyping

b)

Sanger Sequencing

c)

PCR

d)

None of the above

18.

Which of this test is used for detecting chromosomal disorders in fetus?

a)

NIPS

b)

PGS

c)

PGD

d)

Clinical Exome

19.

Which of the following communities is a good example of founder mutation?

a)

The Ashkenazi community

b)

The Bohri Muslim community

c)

The Iyers of Palakkad

d)

The Bhotias of Sikkim

20.

Coding region makes about

a)

10% of the genome

b)

2% of the genome

c)

50% of the genome

d)

100% of the genome