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2_Tools of the Lab Review

Total questions: 14

Worksheet time: 7mins

Name
Class
Date
1.

True or False: Sterile medium contains only one species of microbe and no contaminants.

a)

True

b)

False

2.

Check all the media forms below that contain agar.

a)

Broth

b)

Deep

c)

Slant

d)

Plate

e)

Complex

3.

This type of media is composed of precise amounts of pure chemicals. It is essential when studying nutritional requirements of bacteria.

a)

selective media

b)

differential media

c)

chemically defined media

d)

enrichment culture media

4.

T/F: media can be both complex and defined.

a)

True

b)

False

5.

T/F: The addition of Peptone would make a medium complex.

a)

True

b)

False

6.

This type of media contains specific ingredients that help us to visually distinguish one species of microbe from another.

a)

selective media

b)

differential media

c)

chemically defined media

d)

enrichment culture media

7.

All of the following methods can be used to obtain pure culture EXCEPT:

a)

Streak Plate

b)

Pour Plate

c)

Spread Plate

d)

Smear Plate

8.

The resolution of light microscope is:

a)

1000-2000X

b)

200 nm

c)

0.1 mm

d)

10 nm

9.

Of the microscopes listed below, which would be the best choice for visualizing fine details?

a)

Brightfield

b)

Confocal

c)

Darkfield

d)

Phase Contrast

10.

Which type of microscopes are useful for thick specimens?

a)

Brightfield

b)

Confocal

c)

Darkfield

d)

Fluorescent

e)

Acid-fast

11.

Viruses of a typical size could be viewed with which of the following? (Choose all that apply)

a)

Scanning electron microscope

b)

Transmission electron microscope

c)

Confocal microscope

d)

Phase contrast microscopy

12.

Which of these steps kills microbes and attaches them to a glass slide?

a)

Drying

b)

Heat fixation

c)

Ethanol step

d)

Iodine step

13.

What does Crystal Violet do in the Gram stain?

a)

it stains Gram-positive cells purple

b)

stains all cells purple

c)

stains Gram-negative cells purple

d)

stains all cells pink

14.

At the end of the Acid-Fast stain, positive cells would be stained:

a)

pink by Safranin

b)

red by Carbolfuchsin

c)

blue by methylene blue

d)

green by malachite green