WorksheetsHigher 2.7 - Genetic Control of Metabolism
Total questions: 18
Worksheet time: 9mins
Which of the following is a consequence of mutagenesis?
Mutations will produce an improved strain of a microorganism
Mutations may produce an improved strain of a microorganism
Mutations do not produce an improved strain of a microorganism
Which process involves exposing microorganisms to mutagenic agents?
Mutagenesis
Recombinant DNA Technology
Which process involves transferring genes from one organism into a microorganism?
Mutagenesis
Recombinant DNA Technology
What term is used to describe a combinant of an organisms original DNA and DNA from another source?
Recombinant DNA
Mutagenesis
Vector
Artificial Chromosomes
Which enzyme cuts the desired gene out of the donor DNA?
Restriction endonuclease
Ligase
DNA Polymerase
RNA Polymerase
Which enzyme cuts the plasmid open?
Restriction endonuclease
Ligase
DNA Polymerase
RNA Polymerase
Which enzyme seals the desired gene into a plasmid?
Restriction endonuclease
Ligase
DNA Polymerase
RNA Polymerase
A restriction site is...
A specific target sequence of DNA bases
A general sequence of DNA bases
The area of a plasmid that is cut open
The mutated strands of DNA during mutagenesis
What is the function of a vector?
A DNA molecule used to carry foreign genetic information to another cell
To cut out a specific target sequence of DNA
To seal a desired gene into a plasmid
To protect the microorganism from a selective agent
Which of the following is not an example of a vector?
Plasmid
Artificial Chromosomes
Restriction endonuclease
Which vector is capable of carrying long DNA sequences?
Plasmid
Artificial Chromosomes
What is the role of an origin of replication?
Initiates the self replication of the vector
The target sequence of DNA cut out by restriction endonuclease
Antibiotic resistance genes that protect the microorganism
Controls gene expression
What is the role of regulatory sequences?
Initiates the self replication of the vector
The target sequence of DNA cut out by restriction endonuclease
Antibiotic resistance genes that protect the microorganism
Controls gene expression
What is the role of selectable markers?
Initiates the self replication of the vector
The target sequence of DNA cut out by restriction endonuclease
Antibiotic resistance genes that protect the microorganism
Controls gene expression
How can a selectable marker be used to tell if the host cell has successfully taken up the vector?
Only microorganisms with the vector survive
Microorganisms with and without the vector survive
Only microorganisms without the vector survive
Which of the following is a safety mechanism in recombinant DNA technology?
Adding genes to prevent survival in an external environment
Only cutting out specific target sequences
Using ligase to seal the desired gene into a plasmid
Using recombinant yeast cells
What happens if the 3D structure of recombinant bacteria proteins are incorrectly folded?
The protein is inactive and does not correctly work
The protein is active and works correctly
Which of the following is used to produce correctly folded, more complicated proteins?
Eukaryotic yeast
Prokaryotic bacteria
Prokaryotic archaea
