Free Printable Biotechnolgy Review

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Biotechnolgy Review

Total questions: 17

Worksheet time: 6mins

Name

Class

Date

This is the practice of cutting DNA and separating it by the size of the pieces

Cloning

Gel Electrophoresis

Genetic Engineering

DNA Fingerprinting

Which kitten is most likely the offspring of this cat?

During gel electrophoresis, the _______ pieces of DNA move farthest down the gel

lightest

heaviest

most mutated

deadliest

What does transgenic mean?

An organism that can change its genes at will

A stem cell that can differentiate into anything

An organism that has had another species' DNA put in it

A bacteria that reproduces asexually

What is the purpose of PCR?

To produce millions of copies of DNA.

To produce millions of copies of a specific region of DNA

To add nucleotides to a DNA sequence

To watch polymerase work.

What is the function of a primer?

To identify the particular region of DNA to be copied by PCR.

To copy DNA.

To create DNA nucleotides.

To maintain the temperature of the PCR reaction.

What does the cas9 protein do?

Prevents viruses from entering bacteria

Cut RNA

Cut DNA

Binds to RNA

CRISPR can change DNA in humans and animals. What did scientists look at to help them create CRISPR?

Sheep cloning

Palindromic repeats

Common Cold

Bacteria fighting off viruses

Some restriction enzymes produce "sticky ends". Sticky ends are . . .

single-stranded DNA "overhangs" that can bind to complementary DNA

smooth cuts that are sticky due to the "goo" left behind by the enzyme

The two wires (black and red) on a gel box

The surface of the LB agar plates so that bacteria can grow

10.

What enzyme forms covalent bonds between restriction fragments?

DNA primase

DNA helicase

DNA ligase

DNA polymerase

11.

Restriction Enzymes . . .

cut DNA at precise locations

help bacteria to pick up DNA

move DNA through a gel

copy DNA

12.

You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder.
What PCR "ingredient" would be different in these two tests?

Template DNA

Nucleotides

Taq DNA polymerase

Primers

13.

Which of the following best describes a plasmid?

A section of DNA

Circular DNA found in bacteria

DNA that has genes from different organisms

A bundle of DNA

14.

Which of the following best describes a gene?

A section of DNA

Circular DNA found in bacteria

DNA that has genes from different organisms

A bundle of DNA

15.

Which of the following best describes transgenic organism?

Changing bacterial DNA by adding genes to it

Any organism that has DNA from a different species

Any organism that has DNA from the same species

A tool used by scientists to separate chunks of DNA

16.

Which of the following best describes bacterial transformation?

Changing bacterial DNA by adding genes to it

Any organism that has DNA from a different species

Any organism that has DNA from the same species

A tool used by scientists to separate chunks of DNA

17.

Which of the following best describes a recombinant DNA?

A section of DNA

Circular DNA found in bacteria

DNA that has genes from different organisms

A bundle of DNA

Reset

Biotechnolgy Review

Total questions: 17

Worksheet time: 6mins

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