WorksheetsCRISPR Cas9
Total questions: 9
Worksheet time: 5mins
CRISPR-Cas9 is a type of ________ system found in bacteria.
digestive
endocrine
circulatory
immune
CRISPR-Cas9 was discovered in which of the following organisms?
protists
fungi
bacteria
viruses
Scientists have adapted it to turn it into a biotechnology tool for _______ DNA.
building
editing
destroying
synthesizing
The enzyme portion of this complex is called ________.
CRISPR
Replicase
Lysozyme
Cas9
What type of RNA is used as a GPS of sorts, to find or locate specific genes?
mRNA
gRNA
tRNA
rRNA
What is PAM?
a 3 letter sequence for binding on the target DNA
the main gene portion of the DNA
the cut site for Cas9
a 10 letter sequence for binding on the target DNA
Once the DNA and RNA are bound, Cas9 is activated to cut the DNA ____ upstream from the binding sequence.
1 base
3 bases
6 bases
10 bases
After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following types of repair mechanisms creates a mutation and inactivates the gene.
HDR
NHEJ
After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following repair mechanisms involves "editing" of the gene with insertion of a corrected sequence?
HDR
NHEJ
