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WorksheetsAPPLICATION OF RECOMBINANT DNA
Total questions: 10
Worksheet time: 2mins
A technique for making millions of copies of a specific region of DNA.
DNA ligase
gel electrophoresis
restriction enzymes
polymerase chain reaction
What happens in the Denature step of PCR?
The DNA nucleotides are broken apart.
The double-stranded DNA is separated into two single strands of DNA.
The base-pairing rules for DNA are reversed.
The DNA is returned to its natural setting.
What happens during the Anneal step of PCR?
Primers are created.
Primers copy the new DNA strand
The primers attach to the target DNA region.
Primers sequence DNA
What is the function of a primer?
To identify the particular region of DNA to be copied by PCR.
To create DNA nucleotides
To copy DNA.
To maintain the temperature of the PCR reaction.
What is the purpose of the Extension step of PCR?
To create the primers.
To allow polymerase to create the complementary strands of DNA.
To extend the time it takes to produce DNA.
To allow substrates to copy DNA sequences.
In order to insert a human gene into plasmid, both must
code for the same gene product.
originate from the same type of cell.
be cut by the same restriction enzyme.
have identical sequences.
Recombinant DNA technology
is possible only between closely related species
uses bacteria to make copies of the desired product and splices DNAs together.
uses bacteria to make copies of the desired product
uses bacteria to make copies of the desired product, splices DNAs together, and is possible only between closely related species
The first human hormone produced successfully by using DNA recombinant technology is
human growth hormone
adenosine deaminase
insulin
thyroxin
The following are the uses of
recombinant DNA EXCEPT
Produce sheep clone
Produce plants that have bacterial genes to destroy pathogenic insects
Produce agriculturally important plants and animals
Produce microbes that secrete enzymes to overcome the problems of oil spoilage in the sea
The following are the examples of transgenic organisms EXCEPT
Pseudomonas bacteria strain can breakdown hydrocarbons
Rhizobium bacteria in root nodules that have / the ability to fix nitrogen
sheep that produce milk with human growth hormone
Plants that are resistant to glyphosate
