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Gen Bio 2 -Recombinant DNA

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

Which enzyme could be used to join two DNA fragments?

a)

DNA ligase

b)

DNA polymerase

c)

DNA recombinase

d)

DNA endonuclease

2.

Which statement about recombinant DNA is true?

a)

All restriction endonucleases generate blunt end fragments.

b)

Hydrogen bonds between base pairs are very strong.

c)

Restriction endonucleases cut both strands of DNA at the same time.

d)

Sticky ends prevent DNA ligase activity.

3.

Why are plant cells, as opposed to animal cells, particularly useful as hosts for recombinant DNA?

a)

Animal cells cannot be cultured.

b)

Plant cells can make totipotent stem cells.

c)

Plant cells have reverse transcriptase.

d)

Plant cells have plasmids

4.

Genetic engineering involves

a)

cutting out a DNA sequence.

b)

changing a DNA sequence.

c)

reinserting DNA into living organisms.

d)

all of the above

5.

A recombinant plasmid gets inside a bacterial cell by

a)

. hybridization

b)

radiation

c)

transformation

d)

recombination

6.

A DNA molecule produced by combining DNA from different sources is known as

a)

a mutant

b)

a change

c)

strange DNA

d)

recombinant DNA

7.

The process of making changes in the DNA code of living organisms is called

a)

selective breeding

b)

genetic engineering

c)

inbreeding

d)

hybridization

8.

Small circular pieces of DNA found inside bacteria in addition to their chromosome is called

a)

a DNA

b)

a clone

c)

RNA

d)

a plasmid

9.

What does it mean to “clone a gene”?

a)

make many copies of the gene

b)

remove the gene

c)

change the gene

d)

destroy the gene

10.

DNA from a human has been inserted into a bacterial plasmid and reinserted back into the bacterium. The bacterium now contains both human DNA and bacterial DNA. The bacterium is now considered a

a)

mutation

b)

clone

c)

PCR

d)

transgenic organism