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WorksheetsMicroS03LQ3 - Orthomyxoviridae (Part 3)
Total questions: 11
Worksheet time: 6mins
(Laboratory diagnosis)
Diagnosis of influenza relies on what?
1. Identification of viral Ags or viral nucleic acid in specimens
2. Isolation of the virus
3. Demonstration of a specific immunologic response by patient
1 only
2 only
1 & 2
1, 2, 3
(Laboratory diagnosis)
Nasal washings, gargles, and throat swabs are the specimens needed for the laboratory diagnosis and should be obtained within 3 days after onset of symptoms. Which of the following are the best specimens for diagnostic testing?
Nasopharyngeal swabs
Nasal aspirate
Lavage fluid
All of the above
(Laboratory diagnosis)
It is the preferred for diagnosis of influenza since:
(1) Can detect and distinguish influenza A and B, different strains (e.g., H5N1) and other respiratory viruses
(2) Rapid (<1 day), sensitive and specific
RT-PCR
ELISA
Viral isolation
Culture
(Laboratory diagnosis)
Allow for rapid detection of multiple
pathogens in a single test
Multiplex RT-PCR
ELISA
Viral isolation
Culture
(Laboratory diagnosis)
For viral culture, which usually takes about 3-10 days, isolation methods of choice for influenza viruses commonly uses what?
Embryonated eggs and primary monkey kidney (PMK) cells
Madin-Darby canine kidney cell line
(Laboratory diagnosis)
Sample held at _____°C until inoculation into cell culture since freezing and thawing reduce the ability to recover virus
36
24
12
4
(Laboratory diagnosis)
If storage will exceed 5 days, sample should be frozen at -70°C
True
False
(Laboratory diagnosis)
Commercially available rapid influenza antigen detection tests: Take less than 15 minutes; vary in sensitivity and
specificity, and a negative result does not rule out influenza infection.
True
False
(Management)
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(Management)
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(Management)
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