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MicroS03LQ3 - Orthomyxoviridae (Part 3)

Total questions: 11

Worksheet time: 6mins

Name
Class
Date
1.

(Laboratory diagnosis)


Diagnosis of influenza relies on what?


1. Identification of viral Ags or viral nucleic acid in specimens


2. Isolation of the virus


3. Demonstration of a specific immunologic response by patient

a)

1 only

b)

2 only

c)

1 & 2

d)

1, 2, 3

2.

(Laboratory diagnosis)


Nasal washings, gargles, and throat swabs are the specimens needed for the laboratory diagnosis and should be obtained within 3 days after onset of symptoms. Which of the following are the best specimens for diagnostic testing?

a)

Nasopharyngeal swabs

b)

Nasal aspirate

c)

Lavage fluid

d)

All of the above

3.

(Laboratory diagnosis)

It is the preferred for diagnosis of influenza since:


(1) Can detect and distinguish influenza A and B, different strains (e.g., H5N1) and other respiratory viruses


(2) Rapid (<1 day), sensitive and specific

a)

RT-PCR

b)

ELISA

c)

Viral isolation

d)

Culture

4.

(Laboratory diagnosis)


Allow for rapid detection of multiple

pathogens in a single test

a)

Multiplex RT-PCR

b)

ELISA

c)

Viral isolation

d)

Culture

5.

(Laboratory diagnosis)


For viral culture, which usually takes about 3-10 days, isolation methods of choice for influenza viruses commonly uses what?

a)

Embryonated eggs and primary monkey kidney (PMK) cells

b)

Madin-Darby canine kidney cell line

6.

(Laboratory diagnosis)


Sample held at _____°C until inoculation into cell culture since freezing and thawing reduce the ability to recover virus

a)

36

b)

24

c)

12

d)

4

7.

(Laboratory diagnosis)


If storage will exceed 5 days, sample should be frozen at -70°C

a)

True

b)

False

8.

(Laboratory diagnosis)


Commercially available rapid influenza antigen detection tests: Take less than 15 minutes; vary in sensitivity and

specificity, and a negative result does not rule out influenza infection.

a)

True

b)

False

9.

(Management)


Basahin na lang b hehe

a)

Tapos na

b)

Pindutin yung tapos na

10.

(Management)


Basahin na lang b hehe

a)

Tapos na

b)

Pindutin yung tapos na

11.

(Management)


Basahin na lang b hehe

a)

Tapos na

b)

Pindutin yung tapos na