WorksheetsQS5 Installation Training - Mediven Penang
Total questions: 11
Worksheet time: 6mins
What are the 3 stages of PCR in sequence?
Denaturation, Extension, Annealing
Denaturation, Annealing, Extension
Annealing, Extension, Denaturation
Annealing, Denaturation, Extension
Multiplexing can be performed using SYBR Green assay
True
False
Choose the FALSE statement.
Both PCR and qPCR requires fluorescence dye
qPCR does not require gel electrophoresis
PCR is more time consuming than qPCR
How many VeriFlex zones available in QS5 system?
2
3
4
5
6
What is the maximum zone-to-zone temperature difference for QS5?
5
10
15
Which statement is TRUE about cycle threshold?
Threshold level is above the baseline signal
Cycle threshold is the number of cycle required for fluorescence signal falls below the baseline signal
The lower the Ct value is, the higher concentration of target nucleic acid is in the sample
Threshold is set in exponential phase
Choose the FALSE statement.
Real time PCR requires fluorescence detection
Real time PCR is also known as RT-PCR
A PCR run must always have a NTC control
RNA must be reverse transcribed before PCR
What is the file format of QS5 data file?
.edt
.eda
.edf
.eds
What is the acceptable signal for determining optimal dye concentration in 96-well plate?
400,000
3,000,000
600,000
4,000,000
What is the criteria in successful custom dye calibration?
Uniform signals with peaks aligned with dye's wavelength
Highest dye concentration that falls within the acceptable signal range
How many optical filters does the QS5 instrument have?
4
5
6
