WorksheetsPCR and G/E
Total questions: 15
Worksheet time: 8mins
What does PCR accomplish?
determining the sequence of DNA
develop proteins
make copies of a DNA section
make mRNA
What is the first step of PCR?
termination
denaturation
annealing
splicing
extension
What is the denaturation step?
mutating RNA polymerase
Cooling DNA to allow primers to form
separating DNA helix in a cold environment
separating DNA helix with using heat
At what temperature does the Denature step of PCR occur?
95° C
50° C
72° C
Any temperature
What is the second step of PCR?
denaturation
extension
annealing
termination
splicing
What is the Annealing step?
DNA is cooled to allow primers to form
Heat is used to separate DNA strands
DNA polymerase adds nucleotides to the 5' end
Taq adds nucleotides to the 3' end
At what temperature does the Anneal step of PCR occur?
95° C
50° C
72° C
Any temperature
What is the Extension step?
DNA polymerase adds nucleotides to the 5' end
RNA polymerase adds nucleotides to the 5' end
DNA polymerase adds nucleotides to the 3' end
RNA polymerase adds nucleotides to the 3' end
At what temperature does the Extension step of PCR occur?
95°C
50-65°C
72°C
Any temperature
A student performed a gel electrophoresis experiment. The results are represented in the diagram
below. Compared to the fragments at the top of the gel, the fragments at the lower end are
larger, and move slower
larger, and move faster
smaller, and move faster
smaller, and move slower
