Font size
WorksheetsLABORATORY TOPIC 3: MICROBIAL STERILIZATION AND CONTROL
Total questions: 30
Worksheet time: 20mins
Heated, insulated box used to grow and maintain microbiological or cell cultures:
Autoclave
Oven
Incubator
Water bath
An equipment used to remove microorganisms and spores using high pressure and high temperature steam sterilization:
Water bath
BSCs
Autoclave
Oven
Instrument for very accurate, quantitative mass measurements to the nearest 0.001g:
oven
analytical balance
autoclave
incubator
Provides the highest level of containment for handling the most dangerous microorganisms
BSC Class II
BSC Class I
BSC Class IV
BSC Class III
Mouth pi-petting is strictly performed in the laboratory.
True
False
Plastic or glass pipettes used to transfer small amount of liquid but are not graduated or calibrated for any particular volume.
Mohr
Volumetric
Graduated
Pasteur
CHECK ALL THAT APPLIES:
Correct Objective :
Magnification power pair
OIO:40X
HPO:100X
Scanner:4X
10X:LPO
How to compute for total magnification power?
Oculars / Objective in use
Objective in use - Ocular
Ocular + Objective in use
Ocular X Objective in use
Compute for total magnification:
4x ocular
OIO in use
104x
96x
400x
4000x
Proper conditions for autoclave:
Temperature 121 degrees Celsius
Pressure (a)
Time 15-20mins
The oculars and the eyepiece tube can be found at which part of the microscope?
Base
Arm
Head
None of the above
Which of the following is NOT used to adjust the intensity of light from the microscopic illuminator?
Condenser
Brightness adjustment
Diaphragm
Iris
Arm:
You look through to see the specimen
Holds the slide in place
Used to support the microscope when carried
None of the above
Fine adjustment knob:
Supports the microscope
Moves the stage slightly to sharpen the image
Holds low and high power objective; can be rotated
Holds slide in place
Refers to the hole in the stage of the microscope:
Iris
Stage
Oculars
Aperture
Sterilization is a process that destroys pathogenic organisms but not necessarily all organisms or spores.
True
False
CHECK ALL THAT APPLIES:
Physical methods of disinfection
Filtration
Boiling
UV light
Ionizing radiation
Pasteurization
Under adverse physical and chemical conditions or when nutrients are scarce some bacteria genera and able to form (a) .
The CDC recommends that tabletops be cleaned after blood spills with a (a) dilution of bleach.
Chemical formula of household bleach:
(a)
Which of the following is NOT generally used as a disinfectant due to irritating fumes?
Alcohols
Halogens
Quats
Aldehydes
CHECK ALL THAT APPLIES:
COLD STERILIZATION:
Peracetic acid
amphyl
EtO
Glutaraldehyde
1% silver nitrate
Methods of choice for the sterilization of antibiotic solutions and vaccines:
(a)
Moist heat sterilization requires longer exposure times and higher temperature.
True
False
Used to sterilize HEPA filters in BSCs:
EtO
Formaldehyde vapor
Alcohols
Amphyl
Generally, the time necessary for killing microorganisms (a) in direct proportion to the number of organisms
A solution of (a) is added to the eyes of newborns to prevent neonatal conjunctivitis.
The safest method to assure that no infective materials remain in samples or container when disposed:
ionizing radiation
filtration
pasteurization
incineration
Which of the following is a spore former?
L. monocytogenes
E. coli
C. perfringens
C. diphtheriae
BONUS:
Do you find microbiology interesting? Yes or no?
