WorksheetsFluorimetry PA
Total questions: 10
Worksheet time: 5mins
When molecule absorbed incident electromagnetic radiation it is electron goes excited state. But the electron it is unstable at excited state, this electron will return to ground state. When it ground state it is emitting radiation. This phenomena called ?
A. Fluorescence
B. Phosphorescence
C. A and B
D. None of this
Increase in intensity of light incident on sample increases ________.
A. absorbance
B. wavelength
C. light incident
D. fluorescence intensity
What wavelengths can a Vis Spectroscopy detect?
A. 250-600 nanometers
B. 380-780 nanometers
C. 620-750 nanometers
D. 200 and 800 nanometers
What is the best way to describe fluorimetry ?
General purpose instrument measures voltage, current and resistance.
Device used to measure parameters of visible spectrum fluorescence.
A device capable of counting the individual electrons in an electric current.
Instrument used for detecting and measuring ionizing radiation.
Which spectroscopy is measure intensity of the FLUORESCENCE of molecule?
A. IR
B. NMR
C. Fluorimetry
D. All of the above
Which of the following are used as sources in fluorometry?
i. Deuterium discharge lamp
ii. Incandescent wire
iii. Xenon arc lamp
iv. Mercury vapor lamp
i and ii
i,ii and iii
iii and iv
ii and iv
The purpose of secondary filter in fluorescence spectroscopy is
Allows only emission radiation
Allows only excitation radiation
Allows both excitation and emission radiations
Allows transmitted radiation
What is Jablonski energy Diagram ?
Is a diagram that illustrates the electronic states of a molecule
Its just a diagram.
Diagram too calculate the light
Is the diagram that the states are arranged vertically by energy and grouped horizontally by spin multiplicity.
Which are the variant of Fluorimetry instrument ?
Single beam
Double Beam
Spectrofluorometer
All of the above
How long will light be absorbed when the appropriate frequency is irradiated ?
5-10 seconds
10-15 seconds
Less than 5 second
∞ Infinity ∞
