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Worksheetsr DNA TECH
Total questions: 20
Worksheet time: 10mins
Which of the following is not a cloning vector?
Agrobacterium
Yeast
Neurospora
Bacteriophage
Gene library is
collection of independently isolated DNA of an organism
DNA sequences
variety of genetic characters of an organism
set of books on genes
pBR322 is a plasmid belonging to
yeast
all bacteria
Escherichia coli
bacteriophages
Which of the following probes are most commonly used in Western blotting?
Nucleic acids
Bovine serum albumin
Antibodies
Nonfat dry milk
What is the purpose of "blocking" the nitrocellulose membrane during western blotting?
To encourage non-specific binding
To prevent non-specific binding
To improve binding of secondary antibody
Scientists like extra work
Why are buffers used in Electrophoresis?
Conduct electricity
Remove salts
Add extension energy
Dissolve excess DNA
If proteins are separated according to their electrophoretic mobility then the type of electrophoresis is:
SDS PAGE
Affinity Electrophoresis
Electro focusing
Free flow electrophoresis
Which of the following pairs is INCORRECTLY matched?
Target DNA - gene of interest
Restriction enzyme - Cuts DNA molecule at specific sites
DNA ligase - mapping human chromosomes
Plasmid as a vector - isolated from bacteria
Which of the following is TRUE regarding screening by using antibiotics and X-gal?
I. Antibiotics will prevent the growth of bacteria without plasmid.
II Bacteria cells with recombinant plasmids are resistant to antibiotics and are unable to hydrolyse X-gal.
III. Bacteria cells which contain nonrecombinant plasmids are resistant to antibiotics and are unable to hydrolyse X-gal.
I only.
I and II only.
II and III only.
All of the above.
Below are correct statements regarding a probe EXCEPT
A probe is used to search for its complementary sequence in a sample genome.
The probe is labeled with a radioactive or chemical tag that allows its binding to be visualized
A probe is a single-stranded sequence of DNA or RNA
A probe is a double-stranded sequence of DNA or RNA
In the formation of a cDNA library, RNase used is
RNAse H
RNAse A
Restriction Enzymes are isolated from :
a).Protozoa
b). Bacteria
c).Virus
d).Fungi
Restriction enzymes belong to class of enzymes:
a).Peptidase
b).Amylases
c).Lipases
d).Nucleases
If the target sequence or restriction site is 5'GCTTCG3', what is the complementary sequence read in the 3'-5' direction?
CGAAGC
GCTTCG
CGUUCG
GCUUCG
The unpaired nucleotides produced by the action of restriction enzymes are referred to as.....
sticky end.
base sequence.
single strands.
restriction fragments.
What is a palindrome?
A sticky end resulting from a restriction enzyme
A blunt end resulting from a restriction enzyme
A sequence that reads the same forwards and backwards
A sequence that is complementary to the other
Another name for "recognition site" is...
nucleotide
restriction Site
primer
construction site
Insertional inactivation is defined as
technique to identify desirable gene
technique to recognise non-recombinant cells
technique to recognise recombinant cells
technique to recognise specific cell types
In genetic engineering, the antibiotics are used
as sequences from where replication starts
to keep the cultures free of infection
as selectable markers
to select healthy vectors
