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Worksheets

MOLECULAR BASIS OF INHERITANCE

Total questions: 19

Worksheet time: 10mins

Name
Class
Date
1.

What is the function of primer

a)

To help DNA polymerase for initiation

b)

To help RNA polymerase for initiation

c)

To help DNA polymerase for termination

d)

To help RNA polymerase for termination

2.

DNA replication takes place at _________ phase of the cell

cyle.

a)

G1

b)

S

c)

G2

d)

M

3.

The enzyme DNA dependent RNA polymerase catalyses the polymerisation reaction in ___________direction.

a)

only 5’ → 3’

b)

only 3’ → 5’

c)

both (a) and (b)

d)

none of these

4.

The main enzyme in DNA replication is

a)

DNAse

b)

Proteinase

c)

RNAse

d)

DNA Polymerase

5.

Who proved experimentally that DNA is genetic material?

a)

O. Avery and Colleagues

b)

J. Waston and F. Crick

c)

W. Arber and Colleagues

d)

G. Mendel

6.

The enzyme that restores the phosphodiester linkage between adjacent fragments in the lagging strand during DNA replication is

a)

DNA ligase

b)

primase

c)

reverse transcriptase

d)

helicase

7.

The building blocks for a new DNA molecule are

a)

deoxyribose nucleoside monophosphates

b)

deoxyribose nucleoside diphosphates

c)

deoxyribose nucleoside triphosphates

d)

deoxyribose nucleoside tetraphosphates

8.

The difference between DNA and RNA is that

a)

DNA has thymine and RNA has uracil

b)

DNA has no oxygen bonded to the 2’ carbon; RNA does

c)

DNA is the genetic material; RNA is not.

d)

DNA is double stranded and RNA can’t have hydrogen bond

9.

Which one of the following is not found in DNA?

a)

Carbon

b)

Oxygen

c)

Nitrogen or Hydrogen

d)

Sulfur

10.

The Hershey-Chase experiment

a)

Proved semiconservative replication is the mode for DNA replication

b)

used 32P to label protein

c)

used 35S to label DNA

d)

helped to prove DNA was the genetic molecule

11.

Which of the following types of bacteria were used in Griffith’s transformation experiment?

a)

Diplococcus, R-III and S-II type

b)

Pneumococcus, T2 phage

c)

Streptococcus, R-II and S-III type

d)

Diplococcus, E. coli

12.

In Hershey and Chase experiment, the protein of T2 phage was made radioactive by using

a)

S32

b)

P31

c)

S35

d)

P32

13.

Semiconservative DNA replication was proved by Messelson and Stahl, in which DNA was made

a)

Radioactive using N15

b)

Heavy using 14NH4Cl

c)

Heavy using 15NH4Cl

d)

Radioactive using 14NH4Cl

14.

During DNA replication which of the following does not act as substrates?

a)

dATP

b)

dCTP

c)

dUTP

d)

dGTP

15.

RNA primer is removed by

a)

DNA-P-I

b)

DNAP-II

c)

DNAP-III

d)

Primase

16.

Which of the following acts as substrate as well as provide energy for DNA polymerisation?

a)

Ribonucleoside

b)

Deoxyribonucleoside

c)

Ribonucleotide

d)

Deoxyribonucleoside triphosphate

17.

DNA replication is

a)

Semi-conservative, continuous, unidirectional

b)

Conservative, continuous

c)

Semi-conservative, semi-discontinuous

d)

Semi-continuous, conservative

18.

The leading strand and lagging strand in DNA replication differ in that only on the lagging strand

a)

DNA is replicated as short fragments

b)

RNA primer is present

c)

Replication proceeds in the 5’ to 3’ direction

d)

Okazaki fragments are not needed

19.

In semiconservative replication of DNA

a)

the original double helix remains intact and a new double helix forms

b)

the strands of the double helix separate and act as templates for new strands

c)

polymerization is catalyezed by RNA polymerase

d)

polymerization is catalysed by a double helical enzyme