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Biochemical Pathways

Total questions: 19

Worksheet time: 10mins

Name
Class
Date
1.

What product was produced?

a)

methanol

b)

Ethanol

c)

isopropanol

d)

ethambutol

2.

What is missing

a)

Tryptophan

b)

Pyruvate

c)

Adenylyl cyclase

3.

Major Fermentations, Their End-Products, and Some Organisms That Performed Them:


Alcoholic fermentation

a)

Major End Products: Ethanol and CO2

Representative Organisms: Saccharomyces cerevisiae

b)

Major End Products: Lactate

Representative Organisms: Streptococcus and some

Lactobacillus

c)

Major End Products :Lactate, ethanol, and acetate

Representative Organisms: Streptococcus, Leuconostoc,

and Lactobacillus

d)

Major End Products:2,3-Butanediol

Representative Organisms: Enterobacter, Serratia,

and Erwinia

4.

Major Fermentations, Their End-Products, and Some Organisms That Performed Them:


Homofermentation

a)

Major End Products: Ethanol and CO2

Representative Organisms: Saccharomyces cerevisiae

b)

Major End Products: Lactate

Representative Organisms: Streptococcus and some Lactobacillus

c)

Major End Products: Lactate, ethanol, and acetate

Representative Organisms: Streptococcus, Leuconostoc,

and Lactobacillus

d)

Major End Products: Acetate, formate, succinate,CO2, H2, and ethanol

Representative Organisms: Escherichia, Salmonella,

and ethanol Klebsiella, and Shigella

5.

Major Fermentations, Their End-Products, and Some Organisms That Performed Them:

Heterofermentation

a)

Major End Products: Ethanol and CO2

Representative Organisms: Saccharomyces cerevisiae

b)

Major End Products: Ethanol and CO2

Representative Organisms: Streptococcus and some

Lactobacillus

c)

Major End Products: Lactate, ethanol, and acetate

Representative Organisms: Streptococcus, Leuconostoc,

and Lactobacillus

d)

Major End Products: Acetate, formate, succinate,CO2, H2, and ethanol

Representative Organisms: Escherichia, Salmonella,

and ethanol Klebsiella, and Shigella

6.

Major Fermentations, Their End-Products, and Some Organisms That Performed Them


Mixed acid fermentation

a)

Major End Products: Acetate, formate, succinate, CO2, H2, and ethanol

Representative Organisms: Escherichia, Salmonella, and ethanol Klebsiella, and Shigella

b)

Major End Products: 2,3-Butanediol

Representative Organisms: Enterobacter, Serratia,

and Erwinia

c)

Major End Products: Butanol, butyrate, acetone, and isopropanol

Representative Organisms:Clostridium, Butyrivibrio, and some Bacillus

d)

Major End Products: Propionate, acetate and CO2

Representative Organisms: Propionibacterium, Veillonella,

and some Clostridium

7.

Major Fermentations, Their End-Products, and Some Organisms That Performed Them

2,3-Butanediol fermentation

a)

Major End Products: 2,3-Butanediol

Representative Organisms: Enterobacter, Serratia,

and Erwinia

b)

Major End Products: Butanol, butyrate, acetone, and isopropanol

Representative Organisms: Clostridium, Butyrivibrio,and some Bacillus

c)

Major End Products: Propionate, acetate and CO2

Representative Organisms: Propionibacterium, Veillonella, and some Clostridium

8.

Major Fermentations, Their End-Products, and Some Organisms That Performed Them

Butyrate/butanol fermentation

a)

Major End Products: Propionate, acetate and CO2

Representative Organisms: Propionibacterium, Veillonella,

and some Clostridium

b)

Major End Products: Butanol, butyrate, acetone, and isopropanol

Representative Organisms: Clostridium, Butyrivibrio,and some Bacillus

c)

Major End Products: Lactate, ethanol, and acetate

Representative Organisms: Streptococcus, Leuconostoc,

and Lactobacillus

9.

Major Fermentations, Their End-Products, and Some Organisms That Performed Them

Propionic acid fermentation

a)

Major End Products: Propionate, acetate and CO2

Representative Organisms: Propionate, acetate and CO2

b)

Major End Products: Propionate, acetate and CO2

Representative Organisms: Saccharomyces cerevisiae

c)

Major End Products: Propionate, acetate and

Representative Organisms: Propionibacterium, Veillonella,

and some Clostridium

d)

Major End Products: Propionate, acetate and CO2

Representative Organisms: Enterobacter, Serratia,

and Erwinia

10.

ATP Yields from Complete Oxidation of Glucose to CO2 by a Prokaryote Using Glycolysis, Entry Step, and the Krebs Cycle with O2 as the Final Electron Acceptor

a)

10 NADH+ H are produced with 3 ATP value in the aerobic ETC and a total of 30 ATP per glucose are produced

b)

1 NADH+ H are produced with 4 ATP value in the aerobic ETC and a total of 60 ATP per glucose are produced

c)

100 NADH+ H are produced with 300 ATP value in the aerobic ETC and a total of 3 ATP per glucose are produced

d)

0.1 NADH+ H are produced with 3 ATP value in the aerobic ETC and a total of 300 ATP per glucose are produced

11.

ATP Yields from Complete Oxidation of Glucose to CO2 by a Prokaryote Using Glycolysis,

Entry Step, and the Krebs Cycle with O2 as the Final Electron Acceptor

a)

4 ATP (by substrate ATP phosphorylation) are produce which is equivalent to 4 ATP per glucose molecule

b)

1 ATP (by substrate ATP phosphorylation) are produce which is equivalent to 4 ATP per glucose molecule

c)

4 ATP (by substrate ATP phosphorylation) are produce which is equivalent to 1 ATP per glucose molecule

d)

40 ATP (by substrate ATP phosphorylation) are produce which is equivalent to 4 ATP per glucose molecule

12.

ATP Yields from Complete Oxidation of Glucose to CO2 by a Prokaryote Using Glycolysis, Entry Step, and the Krebs Cycle with O2 as the Final Electron Acceptor

a)

2 FADH(2) are produced with 2 ATP values produced in Aerobic ETC plus a total 4 of ATP per glucose molecule

b)

2 FADH(2) are produced with 1 ATP values produced in Aerobic ETC plus a total 4 of ATP per glucose molecule

c)

2 FADH(2) are produced with 8 ATP values produced in Aerobic ETC plus a total 4 of ATP per glucose molecule

d)

2 FADH(2) are produced with 10 ATP values produced in Aerobic ETC plus a total 4 of ATP per glucose molecule

13.

Entry Step

a)

Reactant: 2 Pyruvates and 2 Coenzyme A

Product : 2 Acetyl CoA2 CO2

2 NAD+ -------------> 2 NADH+2H

b)

Reactant: 2 Acetyl CoA

Product : 4 CO2 and 2 Coenzyme A

6 NAD-------------------->6 NADH+6H

2GDP+2P(=2ADP+2P) ===== GTP or ATP

14.

Krebs Cycle

a)

Reactant: 2 Acetyl CoA

Product : 4 CO2 and 2 Coenzyme A

6 NAD-------------------->6 NADH+6H


2GDP+2P(=2ADP+2P) ===== GTP or ATP

b)

Reactant: 2 Pyruvates and 2 Coenzyme A

Product : 2 Acetyl CoA2 CO2


2 NAD+ -------------> 2 NADH+2H

15.

For every six glucose-6-phosphates that enter and complete the cycle, 6CO2 and 12 NADPH+H

are produced. Some of the five-carbon intermediates,

however, may be redirected into synthesis of aromatic amino acids and nucleotides. If the cycle is performed as shown, 36 carbons enter as six glucose 6-phosphate (6 C6 36C).

a)

PENTOSE-PHOSPHATE CYCLE

b)

ENTNER-DOUDOROFF PATHWAY

16.

Glycolytic Reactants and Products per Glucose


Which is incorrect?

a)

Reactant: Glucose (C6H12O6)

Product: 2 Pyruvates (C3H3O3)

b)

Reactant: 2 ATP

Product: 8 ADP

c)

Reactant: 4 ADP

Product: 4 ATP

d)

Reactant: NET: 2 ADP

Product: NET: 2 ATP

e)

Reactant: 2 NAD

Product: 2 NADH+ 2H

17.

main difference between ENTNER-DOUDOROFF PATHWAY and Glycolysis

a)

the six-carbon compound that is split into two three-carbon compounds. The result of this split

is pyruvate and glyceraldehydes-3-phosphate, which is oxidized as in glycolysis

to pyruvate.

b)

Because only 2 three-carbon compound goes through the sequence of reactions leading to pyruvate, the ATP and NADH yield is one that of glycolysis. But one NADPH is produced that is not made in lipolysis.

c)

the six-carbon compound that is split into four three-carbon compounds. The result of this split

is pyruvate and glyceraldehydes-1-phosphate, which is oxidized as in glycolysis

to pyruvate.

d)

Because only one three-carbon compound goes through the sequence of reactions leading to pyruvate, the ATP and NADH yield is one-half that of glycolysis. But one NADPH is produced that is not made in glycolysis.

18.

It is a complex set of cyclic reactions that provides a mechanism for producing five-carbon sugars (pentoses) from six-carbon sugars (hexoses).

a)

pentose-phosphate pathway

b)

Entner-Doudoroff pathway

19.

It is an alternative means of degrading glucose into

two pyruvates. This pathway is found exclusively among prokaryotes (e.g., Pseudomonas and E. coli, as well as other Gram-negatives and certain Archaea). It allows utilization of a different

category of sugars (aldonic acids) than glycolysis and therefore improves the range of resources available to the organism. It is less efficient than glycolysis because only one ATP is phosphorylated and only one NADH is produced.

a)

Entner-Doudoroff pathway

b)

Endner-Doudoroff pathway

c)

Endner-Duodoroff pathway

d)

phentose-phosphate pathway

e)

phentose-posphate pathway