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WorksheetsChromatography Class test
Total questions: 30
Worksheet time: 17mins
Russian Botanist who developed the technique is referred to as the father of chromatography
Tswet
Goldberg
Waage
Frederic Reuss
Column chromatography is a type of
Adsorption chromatography
Paper chromatography
Partition chromatography
TLC
In Paper chromatography the solvent rises to the top chiefly by
Capillary force
Adsorption
Hydrogen bonding
Passive transport
The Rf value can never be
More than 1
Less than 1
A fraction
Positive
The common solvent for Paper chromatography Butanol: Acetic acid: Water (BAW) , is used in the ratio
4:1:5
4:5:1
6:6:3
6:3:6
Which among the following is the least or weakest polar solvent used in chromatography
Hexane
Acetone
Water
Acetic acid
Which of the following is NOT used as a stationary phase in TLC
Agarose
Silica
Aluminium oxide
Cellulose
A solvent can also be described as
Elutant
Eluent
Column material
Filler
“The higher the number of hydrocarbons in a solvent, the more polar it will be”
False
True
Calcium sulphate is added to stationary phase while preparing the TLC plate is for
Inert binder
Enhancing non polar nature
Decreasing non polar nature
Enhancing water absorption
The manually prepared TLC plate is dried and activated by heating in an oven for thirty minutes at what temperature?>
110 °C
90 °C
70 °C
50 °C
During Gel filtration chromatography, Non spherical proteins are eluted earlier than spherical molecules with the same mass.
True
False
The trade name is Sephadex is actually which one of the following material
Dextran
N,N-methylene bis acrylamide
Agarose
Sepharose
Which among the following is NOT an advantage of Gel filtration chromatography
Do not preserve the biological activity of the particles to be separated.
There is also no sample loss
It doesn’t depend on temperature, pH, ionic strength
All are advantages of GFC
The analysis of non-volatile and thermally-unstable compounds can be done using
HPLC
GC
HTPC
GCC
HPLC can be of
Analytical and Preparative
Analytical and Diagnostic
Analytical and Qualitative
Qualitative and Diagnostic
Normal flow rate in HPLC is
1 to 2 mL/min
5 to 10 mL/min
10 to 20 mL/min
15 to 20 mL/min
Which part of HPLC is called “Heart of chromatograph”
Column
HPLC pump
Detector
Injector device
A stationary phase in GC can be
either solid or liquid
either solid or gas
Solid only
Gas only
Which among the following is never used in gas chromatography as mobile phase
Oxygen
Helium
Nitrogen
hydrogen
In Ion exchange chromatography, the step where all stationary phase charged groups are associated with exchangeable counter ions, such as chloride or sodium, is called
Equilibration
Regeneration
Elution
Ion exchange
Give an example for a Cation exchange resin used in Ion exchange chromatography
Carboxy Methyl Sephadex
Quaternary Ammonium Sephadex
Diethyl aminoethyl Sephadex
Diethyl Ammonium Sephadex
b) Graphite is soluble in water
c) Graphite is insoluble in water
What type of chromatography is depicted by the illustration?
Gel Filtration Chromatography
Column Chromatography
HPLC
Thin Layer Chromatography
The Rf value for the red component is
0.3
0.7
10
7
In HPLC (High Performance Liquid Chromatography) which of these factors does not affect the time taken for a component to pass through the column?
Type of detector
Material of stationary phase
Particle size of stationary phase
Temperature of column
Spot the correct equation
v = ∆E q / 6 π r N d
v = ∆E q / 6 π r n d
v = ∆E q / 6 π r η d
v = ∆E q / 6 π r η v
The staining dye used to detect DNA during gel electrophoresis is
Coomassie brilliant blue
Methylene Blue
Ethidium Bromide
Iodine
Spot the Molecule which is not used for performing Gel Electrophoresis
N N’ methyl tri acrylamide
Acrylamide Polymer
Tetramethylene diamine (TEMED),
Ammonium per Hydrate (APH)
Name the type of chromatography used to detect adulterants in food, pesticides in drinking water, poison in liver specimens ?
(a)
