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Biotechnology

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

The first genomic libraries were cloned in _______________

a)

Plasmid

b)

Bacteria

c)

Human

d)

Plants

2.

Genomic library construction is concerned with ___________

a)

Gene isolation

b)

Protein production

c)

Antibiotics

d)

Regeneration

3.

A genomic library is a collection of _____________

a)

Genes

b)

Proteins

c)

Vectors

d)

Recombinants

4.

The Klenow fragment is basically a

a)

DNA hybrid

b)

DNA polymerase

c)

RNA polymerase

d)

Promoter

5.

Which organism has the highest number of vectors?

a)

Yeast

b)

Mammalian cells

c)

E.coli

d)

Fungi

6.

Which is an example of a simplest vector (in terms of size)?

a)

2 micron circle

b)

Bacteriophage

c)

Plasmid

d)

YAC

7.

Which antibiotic resistance is present in pBR322?

a)

Ampicillin

b)

Kanamycin

c)

Lactase

d)

Gentamycin

8.

Chromosomal or genomic DNA is separated by

a)

Sedimentation

b)

Dissolution in water

c)

Centrifugation

d)

Distillation

9.

Proteins can be removed via treatment by?

a)

Treatment with sodium hydroxide

b)

Phenol and chloroform treatment

c)

Chloroform treatment alone

d)

Centrifuging

10.

Cell lysis is carried out by which substance?

a)

Water

b)

Lysozyme and detergents

c)

Suphuric Acid

d)

Sugar solution