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WorksheetsIsolation of Pure Culture C35&36
Total questions: 10
Worksheet time: 30mins
The followings are the importance of culture methods EXCEPT.
To isolate bacteria in pure cultures.
To determine insensitivity to antibodies.
To maintain stock cultures.
To estimate viable counts.
At which quadrant we expect to find isolated bacterial colony in streak method?
Quadrant 1
Quadrant 2
Quadrant 3
Quadrant 4
Why do we need to flame the inoculating loop in each streak?
To increase the bacterial population.
To achieve viable counts of bacteria
To dilute the bacterial population
To sterilize the agar plate
From practical 2 lecture video, what type of media transfer was conducted?
Agar plate to agar plate
Broth to agar plate
Broth to slant agar
Slant agar to Broth media
According to lecture video practical 2, what bacteria is used in the 4 streak technique?
Escherichia coli
Streptococcus pyogenes
Staphylococcus aureus
Pseudomonas aeruginosa
The followings are objectives of serial dilutions EXCEPT.
To make viable colony counts.
To produce colony forming units.
To dilute the bacterial population.
To understand antibacterial sensitivity.
Which of the followings is the optimum number of colonies to make viable bacterial count?
10-100 colonies
20-200 colonies
20-400 colonies
10-300 colonies
According to lecture video practical 3, what is the volume pipeted from the micropipette onto the agar plate?
1000 microlitre
0.1 mililitre
100 microlitre
0.11 mililitre
What hazard we want to avoid when flaming the 'hockey stick' spreader before spreading onto the agar plate?
Define pure culture.
