Font size
WorksheetsGene cloning revision (1)
Total questions: 11
Worksheet time: 8mins
The diagram shows the structure of E.coli, which is gram negative. How can the cell extract be obtained?
Add EDTA, lysozyme, and SDS.
Add lysozyme and SDS.
Add EDTA and SDS.
Add EDTA and lysosyme.
From the diagram, we can conclude that...
low salt concentration causes DNA to elute from the column.
DNA binds most tightly to the ion-exchange resin.
RNA and protein bind most tightly to the ion-exchange resin.
a single salt concentration can separate DNA from RNA and protein.
This diagram shows...
how plasmids are separated from chromosomal DNA by size
the alkaline denaturation method
how plasmids are separated from chromosomal DNA by conformation
all of the above
this diagram shows the action of...
terminal transferase
alkaline phosphatase
polynucleotide kinase
the klenow fragment
in the diagram shown, what is enzyme A?
DNA ligase
DNA polymerase II
Klenow fragment
Taq polymerase
in homopolymer tailing using terminal transferase, the issues shown may result. how can these be solved?
ligase and DNA polymerase I
klenow fragment and restriction endonuclease
restriction endonuclease and polynucleotide kinase
ligase and klenow fragment
How are deoxyribonucleotides different from dideoxyribonucleotides? Choose all that may apply.
Deoxyribonucleotides are used in both PCR and Cycle (Chain termination) Sequencing.
Dideoxyribonucleotides are missing an OH group compared to deoxyribonucleotides.
You cannot add new nucleotides to a deoxyribonucleotide.
Deoxyribonucleotides are NOT found in natural living cells.
Dideoxyribonucleotides are labeled with fluorescent tags.
How is DNA synthesis in PCR and Sanger's Chain Termination Sequencing the SAME?
Both require DNA polymerase.
Both require primers.
Both require deoxynucleotides (dNTPs).
Both require dideoxynucleotides (dNTPs).
Both generate new DNA products of equal length.
How is DNA synthesis in PCR and Sanger's Chain Termination Sequencing DIFFERENT?
Only PCR uses deoxynucleotides.
Cycle Sequencing generates DNA products of different lengths.
Only PCR analyzes DNA products using gel electrophoresis.
Only Cycle Sequencing uses dideoxynucleotides.
What do dideoxynucleotides do when added to a growing DNA strand during Sanger's Chain Termination Sequencing?
They stop the elongation of that strand.
That double the length of that strand.
They shorten the length of the strand.
They denature the strand.
What is needed for both the Sanger method and Pyrosequencing?
labelled nucleotides
single stranded DNA
bases that terminate the chain
ATP sulfurylase
