NEW
Font size
Worksheets1.3.1 DNA Detectives
Total questions: 14
Worksheet time: 21mins
In Part I DNA Digestion with Restriction Enzymes
If I'm going to do a 30-60 minute restriction digestion, what temp should my water bath be?
37 degrees C
45 degrees C
37 degrees F
45 degrees F
In Part I DNA Digestion with Restriction Enzymes
How many tubes does your team need to label for this lab?
1
2
3
4
In Part I DNA Digestion with Restriction Enzymes
What do you add to your tubes RIGHT BEFORE freezing them?
SYBRSafe
10x gel loading dye (solution)
enzyme
DNA
In the gel making video, when making a 30 ml gel, what mass of agarose powder should be added to the buffer to make the gel?
0.24g
0.48g
.008g
24g
In Part I DNA Digestion with Restriction Enzymes
How much Enzyme 1 will you need TOTAL for this lab (this includes for both Missing Person 1 and Missing Person 2)
15 microliters
25 microliters
30 microliters
45 microliters
What's the purpose of adding the restriction enzymes to your sample? (Remember what restriction enzymes do from the Bozeman Video)
they'll separate the DNA bases
they'll copy the DNA
they'll cut the DNA
they'll make the DNA glow
From your gel making video notes, what role will SYBR Safe play in this lab?
It will make the DNA strands move
It will make the DNA strands separate
It will copy the DNA strands
It will make the DNA strands visible
From the gel making video, why do we wrap the gel in aluminum foil after making it?
to prevent light from damaging the stain
to keep it moist
to allow the DNA strands to be copied
to hide it from other teams
What's the purpose of gel electrophoresis?
(Check your Bozeman Video notes)
to make the DNA visible
to separate the pieces of DNA
to tag the DNA pieces with color
to copy the pieces of DNA
Part 2 Gel Electrophoresis of Restriction Enzymes
When loading your gel, what should go into lane 6?
(MP=Missing Person, E=Enzyme)
MP 1 cut with E1
MP 1 cut with E2
MP 2 cut with E1
MP 2 cut with E2
In the Gel Loading Video, which well comb should you use when making your gel?
6 wells
8 wells
12 wells
16 wells
Part 2 Gel Electrophoresis of Restriction Fragments
Which voltage should we use to run our gel?
75 V
100 V
125 V
150 V
Day 2 Gel Electrophoresis of Restriction Enzymes
In order to make sure you're able to get ALL of your sample out, you should 1st ____ your tubes
freeze
centrifuge (spin)
microwave
Part 2 Gel Electrophoresis of Restriction Enzymes
How much of each sample should you load?
15 microliters
20 microliters
30 microliters
45 microliters
