WorksheetsMolecular Biology Quiz Buster 2
Total questions: 25
Worksheet time: 13mins
Damage of ancient DNA can be caused by
a. Chemicals
b. halflife degradation
c. base changes
d. heat
e. All of the Above
It is theorized that Homo sapiens inherited many detrimental genes from interbreeding with Neanderthals.
True
False
Which of the following is not correctly paired:
a. Haplotype – Represents all SNPs in a set of alleles for each individual
b. Phenotype – Observable traits, characteristics, physical properties of an organism
c. Wildtype – The standard, normal, or non-mutated version of a gene
d. Karyotype – The set of all genes on a particular chromosome
Crispr cleavage depends on:
a. PAM sequence
b. Kozak sequence
c. Shine-Dalgarno sequence
d. UTR sequence
When considering CRIPSR which of the following is false?
a. Since humans are multicellular there is limited ability to be used for gene editing currently.
b. What editing can take place in humans ideally must be done during embryogenesis to have significant long-term effects.
c. Off-targets must carefully be considered so the Cas9 editing does not cause unexpected mutations elsewhere in the genome.
d. CRISPR is a naturally occurring complex in humans that simply requires the application of guide RNA to carryout editing.
RNA Processing involves all of the following except?
a. 5’ Capping
b. 3’ Polyadenylation
c. Reverse Transcription
d. Intron Splicing
Alternative splicing enables all of the following except?
a. Increase in complexity of the genome
b. conservation of genomic space
c. Increase in protein diversity
d. Exon reshuffling
e. None of the above
A specific gene for Glucose metabolism exists in mice and humans making it a(n)?
a. Ortholog
b. Paralog
c. Operon
d. Integral
Genomics involving observations of the similarities and differences of healthy and disease states or contemporary and ancestor genomes is likely:
a. Structural
b. Comparative
c. Functional
d. Epigenetics
When sequencing a novel sample of ancient DNA the optimal sequencing technique would be:
a. Sanger
b. Next Gen
c. Nanopore
d. Whole Genome
Given the above table of data what can be concluded?
a. Disease is 9 times higher if G is present
b. Disease is 6 times higher if T is present
c. Disease is 4 times less likely if T is present
d. There is no correlation between allele G and Disease
When considering Knudson’s 2 hit hypothesis the most applicable type of gene is a(n):
a. oncogene
b. Tumour Supressor
c. homolog
d. Response Element
Non-homologous end joining will result in random mutations unless:
a. Donor DNA is present
b. p53 is expressed
c. cmyc is expressed
d. There is another allele available to maintain wildtype function
An example of a Driver Mutation could be
a. B4LD gene causing loss of hair
b. p53 gene causing loss of control over cell cycle
c. Ras gene causing loss of taste
d. C-Myc gene causing loss of teeth
Cancer mutations in regards to Tumour suppressors can arise due to:
a. Loss of Heterozygosity
b. Haploinsufficiency
c. mutant homozygosity
d. All of the above
Given the Tumour suppressor RB1 which of the following could lead to cancer development:
a. RB1 (WT/WT)
b. RB1 (WT/Mutant)
c. RB1 (Mutant/Mutant)
d. Both b and c
Oncogenes only require only hit to drive cancerous events while Tumour Suppressor genes can require two.
True
False
Crispr is naturally occurring in some bacteria as an antibiotic measure.
True
False
When dealing with Ancient DNA, damage is typically caused immediately in the wake of death rather than over millennia.
True
False
When progressing from a benign to malignant tumour in colorectal cancer a key factor is:
a. invasion or formation of blood vessels
b. accumulation of passenger mutations
c. loss of tumour suppressor gene APC
d. All of the Above
When designing a forward primer with a C-Terminal tag you must include a Kozak Sequence.
True
False
When designing a forward primer with an N-Terminal tag you must check the reading frame.
True
False
When there is a C-Terminal tag you must include a Stop codon in the reverse primer.
True
False
Three main classes of Ribozymes include all except:
a. Self-splicing introns
b. Restriction Enzymes
c. self-cleaving RNA
d. Ribonuclease
When considering the principle of Co-linearity which of the following apply:
a. A continuous sequence of DNA encodes a continuous proportional sequence of amino acids
b. It holds for some prokaryotes
c. Broken by the 5’/3’ UTRs
d. All of the above
