NEW
Font size
WorksheetsMicrobiology Exam 2 Part 1 (Chapter 6 and 9)
Total questions: 65
Worksheet time: 33mins
Which of the following are the top 5 most abundant elements found in living organisms?
Fe, Ca, Mg, H, N
P, S, K, N, H
K, Na, C, O, Cl
C, O, N, H, P
Which of the following are NOT indicated to be essential for humans?
Fe
Se
Co
Au
A substance normally cannot cross the cell membrane. There concentration of this substance is 10 times higher in the outside of the cell. To import this substance from the outside of the cell to the inside, the cell would carry out:
Active transport
Simple Diffusion
Facilitated Diffusion
Both active transport and simple diffusion
Plant cell in hypotonic solution
Plasolysed
Stays Turgid
flaccid (Not normal)
Burst Lyses
Becomes shriveled/shrunken
Plant cell in Hypertonic Solution
Plasolysed
Stays Turgid
flaccid (Not normal)
Burst Lyses
Becomes shriveled/shrunken
Plant cell in Isotonic Solution
Plasolysed
Stays Turgid
flaccid (Not normal)
Burst Lyses
Becomes shriveled/shrunken
Animal cell in hypotonic Solution
Plasolysed
Stays Turgid
flaccid (Not normal)
Burst Lyses
Becomes shriveled/shrunken
Animal cell in hypertonic Solution
Plasolysed
Stays Turgid
flaccid (Not normal)
Burst Lyses
Becomes shriveled/shrunken
Animal cell in isotonic Solution
Plasolysed
Stays Turgid
Stays Normal
Burst Lyses
Becomes shriveled/shrunken
Sodium-Potassium Pumps
pumps 2Na+ out and 3K+ in about once an hour, without the use of energy
pumps 3Na+ out and 2K+ in about once an hour, without the use of energy
continuously pumps 2Na+ out and 3K+ in, powered by ATP
pumps 2Na+ out and 2K+ in, powered by ATP hydrolysis
continuously pumps 3Na+ out and 2K+ in, powered by ATP
Which of the following will be imported by receptor-mediated endocytosis by a cell?
H2O
Glucose
LDL vesicles in the blood
CO2
O2
Psychrophile
optimum temperature around 10-20 C; usually doesn't grow below freezing temperature
optimum growth around 25 to 40 C
optimum growth temperature around 0 C; can growth below freezing temperature
optimum growth around 65-70 C
can actively grow above the boiling point of water
mesophile
optimum temperature around 10-20 C; usually doesn't grow below freezing temperature
optimum growth around 25 to 40 C
optimum growth temperature around 0 C; can growth below freezing temperature
optimum growth around 65-70 C
can actively grow above the boiling point of water
psychrotroph
optimum temperature around 10-20 C; usually doesn't grow below freezing temperature
optimum growth around 25 to 40 C
optimum growth temperature around 0 C; can growth below freezing temperature
optimum growth around 65-70 C
can actively grow above the boiling point of water
thermophile
optimum temperature around 10-20 C; usually doesn't grow below freezing temperature
optimum growth around 25 to 40 C
optimum growth temperature around 0 C; can growth below freezing temperature
optimum growth around 65-70 C
can actively grow above the boiling point of water
extreme thermophile
optimum temperature around 10-20 C; usually doesn't grow below freezing temperature
optimum growth around 25 to 40 C
optimum growth temperature around 0 C; can growth below freezing temperature
optimum growth around 65-70 C
can actively grow above the boiling point of water
The enzyme that can convert singlet oxygen to hydrogen peroxide is called:
DNAses
Hydrogen Synthase
Amylase
Catalase
Superoxide Dismutase
The enzyme that can breakdown Hydrogen Peroxide to water and Oxygen Gas is called:
Hydrogen Synthase
Amylase
Lipases
Catalase
Superoxide Dismutase
Obligate Aerobe
Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence
Do not require oxygen for metabolism, but use it when it is present.
oxygen is necessary for metabolism and growth
Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism
Do not utilize oxygen but can survive and grow to a limited extent in its presence
microaerophiles
Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence
Do not require oxygen for metabolism, but use it when it is present.
oxygen is necessary for metabolism and growth
Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism
Do not utilize oxygen but can survive and grow to a limited extent in its presence
Facultative anaerobes
Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence
Do not require oxygen for metabolism, but use it when it is present.
oxygen is necessary for metabolism and growth
Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism
Do not utilize oxygen but can survive and grow to a limited extent in its presence
Obligate Anaerobes
Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence
Do not require oxygen for metabolism, but use it when it is present.
oxygen is necessary for metabolism and growth
Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism
Do not utilize oxygen but can survive and grow to a limited extent in its presence
E. coli grows in your intestine and produces vitamin K for you to help blood clotting. What relationship does it have with you when it is residing in your gut?
Synergistic relationship
Parasitic relationship
Commensalistic Relationship
Antagonistic Relationship
Mutualistic Relationship
Where would you find biofilms?
a wet sink
in your toothbrush
In your teeth
a kitchen sponge
All of the choices shown
If a bacterium doubles every 30 mins, starting with one cell, after 2 hours, you expect to find:
4 cells
16 cells
8 cells
1 cell
6 cells
A continuous culture is grown in a growth chamber called:
active growth chamber
beaker
thermostat
chemostat
rheostat
Which of the following can be used for quantifying the number of bacteria in a culture?
Direct Cell count under a microscope
Flow Cytometry
Turbidity (optical density) measurement
all of the choices shown
Lag Phase
Cell begins to die at an exponential rate
Cell birth and cell death rates are equal
Cells are not yet multiplying at their maximum rate
Growth increases geometrically
Lag Phase (for exponential growth phase)
Cell begins to die at an exponential rate
Cell birth and cell death rates are equal
Cells are not yet multiplying at their maximum rate
Growth increases geometrically
Stationary Phase
Cell begins to die at an exponential rate
Cell birth and cell death rates are equal
Cells are not yet multiplying at their maximum rate
Growth increases geometrically
Death Phase
Cell begins to die at an exponential rate
Cell birth and cell death rates are equal
Cells are not yet multiplying at their maximum rate
Growth increases geometrically
Sterilization
Complete destruction of all living organisms including endospores
Destruction of all vegetative cells, except for endospores
Reduction in Microbial Count
Reduction in microbial count on skin or mucous membrane
Disinfection
Complete destruction of all living organisms including endospores
Destruction of all vegetative cells, except for endospores
Reduction in Microbial Count
Reduction in microbial count on skin or mucous membrane
Decontamination/Sanitization
Complete destruction of all living organisms including endospores
Destruction of all vegetative cells, except for endospores
Reduction in Microbial Count
Reduction in microbial count on skin or mucous membrane
Antisepsis
Complete destruction of all living organisms including endospores
Destruction of all vegetative cells, except for endospores
Reduction in Microbial Count
Reduction in microbial count on skin or mucous membrane
Which of the following groups of pathogens are usually the most susceptible to antimicrobial agents?
Gram negative bacteria
Endospores
Gram Positive Bacteria
Fungi
Enveloped Viruses
Which of the following groups of pathogens are usually the least susceptible to antimicrobial agents?
mycobacteria
Endospores
Cysts of Protozoa
Fungi
Enveloped Viruses
Which of the following is the strongest antimicrobial agents?
Virucidal Liquid
Spermidicial Liquid
Sporidicial Liquid
Fungicidal Liquid
Bacteriostatic Liquid
Which of the following CANNOT be used as antiseptic?
70% alcohol
Crystal Violet
Hydrogen Peroxide
Iodine
Glutaraldehyde
Reduction in bacterial count from 107 to 103 (4 log drop) results in ____________ death
40%
99.99%
100%
90%
30%
Which of the following can disrupt the cell membrane integrity to cause leakage ?
cholesterol
100% alcohol
distilled water
70% alcohol and detergent
detergent only
Moist heat (such as an autoclave) conditions that can sterilize is:
60 C for 30 min
141 C for 1 second
100 C for 3 Hours
121 C for 15 min
70 C for 15 seconds
Dry heat conditions that can sterilize is:
100 C for 2 Hours
170 C for 60 min
70 C for 2 min
121 C for 15 min
141 C for 10 min
Autoclave
121 C for 15 min
72 C for 15 seconds
140 C for 1 seconds
100 C steam for 30-60 mins
Exposing to a vacuum under freezing conditions
Pasteurization
121 C for 15 min
72 C for 15 seconds
140 C for 1 seconds
100 C steam for 30-60 mins
Exposing to a vacuum under freezing conditions
Ultrapasteurization/UHT
121 C for 15 min
72 C for 15 seconds
140 C for 1 seconds
100 C steam for 30-60 mins
Exposing to a vacuum under freezing conditions
Tyndallization
121 C for 15 min
72 C for 15 seconds
140 C for 1 seconds
100 C steam for 30-60 mins
Exposing to a vacuum under freezing conditions
Lypholization
121 C for 15 min
72 C for 15 seconds
140 C for 1 seconds
100 C steam for 30-60 mins
Exposing to a vacuum under freezing conditions
Pasteurization kills 100% of pathogens from drinks
True
False
Which of the following does NOT cause direct DNA strand breaks?
x rays
cathode rays
gamma rays
ultraviolet light
Which of the following may intentionally be used by scientists to enhance the mutation rates of bacteria?
cathode rays
uv light
x rays
gamma rays
The size of a filter that can filter out bacterial endospore is:
2mm
10um
0.2mm
2000nm
0.2 um (micrometer) or 200 nm
Which of the following is the most effective in killing bacteria?
3% hydrogen peroxide
soap
70% alcohol
100% alcohol
detergent
Which is the following used to be very common in "total toothpaste" and antibacterial soap but now banned ?
Sodium bi-carbonate
Peroxide
Benzalkonium Chloride
Triclosan
Alcohol
The complex ionic compound found in antibacterial wipes is usually:
dimethyl benzyl ammonium chloride (benzalkonium chloride)
eucalyptus oil
20% alcohol
triclosan
Which concentration of alcohol is the most effective in killing bacteria?
21.6%
70%
100%
30%
Which of the following is found is "Peridex" mouthwash?
0.12% chlorhexidine gluconate
70% alcohol
3% hydrogen peroxide
benzalkonium chloride
Which of the following is commonly used for treating burned skins?
70% alcohol
10% bleach
3% hydrogen peroxide
silver sulfadiazaine
4% formalin
Which of the following is commonly added to soft drinks as a preservative?
Chlorhexidine Gluconate
Citric Acid
Ascorbic Acid
Benzalkonium Chloride
Benzoic or sorbic acid
drop from 10^9 to 10^5
90%
99%
99.9%
99.99%
99.999%
drop from 10^7 to 10^2
90%
99%
99.9%
99.99%
99.999%
drop from 10^8 to 10^7
90%
99%
99.9%
99.99%
99.999%
drop from 10^9 to 10^3
90%
99%
99.99%
99.999%
99.9999%
drop from 10^6 to 10^3
90%
99%
99.9%
99.999%
99.9999%
Aerotolerant anaerobes
Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence
Do not require oxygen for metabolism, but use it when it is present.
oxygen is necessary for metabolism and growth
Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism
Do not utilize oxygen but can survive and grow to a limited extent in its presence
