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Microbiology Exam 2 Part 1 (Chapter 6 and 9)

Total questions: 65

Worksheet time: 33mins

Name
Class
Date
1.

Which of the following are the top 5 most abundant elements found in living organisms?

a)

Fe, Ca, Mg, H, N

b)

P, S, K, N, H

c)

K, Na, C, O, Cl

d)

C, O, N, H, P

2.

Which of the following are NOT indicated to be essential for humans?

a)

Fe

b)

Se

c)

Co

d)

Au

3.

A substance normally cannot cross the cell membrane.   There concentration of this substance is 10 times higher in the outside of the cell.  To import this substance from the outside of the cell to the inside, the cell would carry out:

a)

Active transport

b)

Simple Diffusion

c)

Facilitated Diffusion

d)

Both active transport and simple diffusion

4.

Plant cell in hypotonic solution

a)

Plasolysed

b)

Stays Turgid 

c)

flaccid (Not normal)

d)

Burst Lyses

e)

Becomes shriveled/shrunken

5.

Plant cell in Hypertonic Solution

a)

Plasolysed

b)

Stays Turgid 

c)

flaccid (Not normal)

d)

Burst Lyses

e)

Becomes shriveled/shrunken

6.

Plant cell in Isotonic Solution

a)

Plasolysed

b)

Stays Turgid 

c)

flaccid (Not normal)

d)

Burst Lyses

e)

Becomes shriveled/shrunken

7.

Animal cell in hypotonic Solution

a)

Plasolysed

b)

Stays Turgid 

c)

flaccid (Not normal)

d)

Burst Lyses

e)

Becomes shriveled/shrunken

8.

Animal cell in hypertonic Solution

a)

Plasolysed

b)

Stays Turgid 

c)

flaccid (Not normal)

d)

Burst Lyses

e)

Becomes shriveled/shrunken

9.

Animal cell in isotonic Solution

a)

Plasolysed

b)

Stays Turgid 

c)

Stays Normal

d)

Burst Lyses

e)

Becomes shriveled/shrunken

10.

Sodium-Potassium Pumps

a)

pumps 2Na+ out and 3K+ in about once an hour, without the use of energy

b)

pumps 3Na+ out and 2K+ in about once an hour, without the use of energy

c)

continuously pumps 2Na+ out and 3K+ in, powered by ATP

d)

pumps 2Na+ out and 2K+ in, powered by ATP hydrolysis

e)

continuously pumps 3Na+ out and 2K+ in, powered by ATP

11.

Which of the following will be imported by receptor-mediated endocytosis by a cell?

a)

H2O

b)

Glucose

c)

LDL vesicles in the blood

d)

CO2

e)

O2

12.

Psychrophile

a)

optimum temperature around 10-20 C; usually doesn't grow below freezing temperature

b)

optimum growth around 25 to 40 C

c)

optimum growth temperature around 0 C; can growth below freezing temperature

d)

optimum growth around 65-70 C

e)

can actively grow above the boiling point of water

13.

mesophile

a)

optimum temperature around 10-20 C; usually doesn't grow below freezing temperature

b)

optimum growth around 25 to 40 C

c)

optimum growth temperature around 0 C; can growth below freezing temperature

d)

optimum growth around 65-70 C

e)

can actively grow above the boiling point of water

14.

psychrotroph

a)

optimum temperature around 10-20 C; usually doesn't grow below freezing temperature

b)

optimum growth around 25 to 40 C

c)

optimum growth temperature around 0 C; can growth below freezing temperature

d)

optimum growth around 65-70 C

e)

can actively grow above the boiling point of water

15.

thermophile

a)

optimum temperature around 10-20 C; usually doesn't grow below freezing temperature

b)

optimum growth around 25 to 40 C

c)

optimum growth temperature around 0 C; can growth below freezing temperature

d)

optimum growth around 65-70 C

e)

can actively grow above the boiling point of water

16.

extreme thermophile

a)

optimum temperature around 10-20 C; usually doesn't grow below freezing temperature

b)

optimum growth around 25 to 40 C

c)

optimum growth temperature around 0 C; can growth below freezing temperature

d)

optimum growth around 65-70 C

e)

can actively grow above the boiling point of water

17.

The enzyme that can convert singlet oxygen to hydrogen peroxide is called:

a)

DNAses

b)

Hydrogen Synthase

c)

Amylase

d)

Catalase

e)

Superoxide Dismutase

18.

The enzyme that can breakdown Hydrogen Peroxide to water and Oxygen Gas is called:

a)

Hydrogen Synthase

b)

Amylase

c)

Lipases

d)

Catalase

e)

Superoxide Dismutase

19.

Obligate Aerobe

a)

Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence

b)

Do not require oxygen for metabolism, but use it when it is present.

c)

oxygen is necessary for metabolism and growth

d)

Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism

e)

Do not utilize oxygen but can survive and grow to a limited extent in its presence

20.

microaerophiles

a)

Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence

b)

Do not require oxygen for metabolism, but use it when it is present.

c)

oxygen is necessary for metabolism and growth

d)

Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism

e)

Do not utilize oxygen but can survive and grow to a limited extent in its presence

21.

Facultative anaerobes

a)

Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence

b)

Do not require oxygen for metabolism, but use it when it is present.

c)

oxygen is necessary for metabolism and growth

d)

Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism

e)

Do not utilize oxygen but can survive and grow to a limited extent in its presence

22.

Obligate Anaerobes

a)

Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence

b)

Do not require oxygen for metabolism, but use it when it is present.

c)

oxygen is necessary for metabolism and growth

d)

Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism

e)

Do not utilize oxygen but can survive and grow to a limited extent in its presence

23.

E. coli grows in your intestine and produces vitamin K for you to help blood clotting.  What relationship does it have with you when it is residing in your gut?

a)

Synergistic relationship

b)

Parasitic relationship

c)

Commensalistic Relationship

d)

Antagonistic Relationship

e)

Mutualistic Relationship

24.

Where would you find biofilms?

a)

a wet sink

b)

in your toothbrush

c)

In your teeth

d)

a kitchen sponge

e)

All of the choices shown

25.

If a bacterium doubles every 30 mins, starting with one cell, after 2 hours, you expect to find:

a)

4 cells

b)

16 cells

c)

8 cells

d)

1 cell

e)

6 cells

26.

A continuous culture is grown in a growth chamber called: 

a)

active growth chamber

b)

beaker

c)

thermostat

d)

chemostat

e)

rheostat

27.

Which of the following can be used for quantifying the number of bacteria in a culture?

a)

Direct Cell count under a microscope

b)

Flow Cytometry

c)

Turbidity (optical density) measurement

d)

all of the choices shown

28.

Lag Phase

a)

Cell begins to die at an exponential rate

b)

Cell birth and cell death rates are equal

c)

Cells are not yet multiplying at their maximum rate

d)

Growth increases geometrically

29.

Lag Phase (for exponential growth phase)

a)

Cell begins to die at an exponential rate

b)

Cell birth and cell death rates are equal

c)

Cells are not yet multiplying at their maximum rate

d)

Growth increases geometrically

30.

Stationary Phase

a)

Cell begins to die at an exponential rate

b)

Cell birth and cell death rates are equal

c)

Cells are not yet multiplying at their maximum rate

d)

Growth increases geometrically

31.

Death Phase

a)

Cell begins to die at an exponential rate

b)

Cell birth and cell death rates are equal

c)

Cells are not yet multiplying at their maximum rate

d)

Growth increases geometrically

32.

Sterilization

a)

Complete destruction of all living organisms including endospores

b)

Destruction of all vegetative cells, except for endospores

c)

Reduction in Microbial Count

d)

Reduction in microbial count on skin or mucous membrane

33.

Disinfection

a)

Complete destruction of all living organisms including endospores

b)

Destruction of all vegetative cells, except for endospores

c)

Reduction in Microbial Count

d)

Reduction in microbial count on skin or mucous membrane

34.

Decontamination/Sanitization

a)

Complete destruction of all living organisms including endospores

b)

Destruction of all vegetative cells, except for endospores

c)

Reduction in Microbial Count

d)

Reduction in microbial count on skin or mucous membrane

35.

Antisepsis

a)

Complete destruction of all living organisms including endospores

b)

Destruction of all vegetative cells, except for endospores

c)

Reduction in Microbial Count

d)

Reduction in microbial count on skin or mucous membrane

36.

Which of the following groups of pathogens are usually the most susceptible to antimicrobial agents?

a)

Gram negative bacteria

b)

Endospores

c)

Gram Positive Bacteria

d)

Fungi

e)

Enveloped Viruses

37.

Which of the following groups of pathogens are usually the least susceptible to antimicrobial agents?

a)

mycobacteria

b)

Endospores

c)

Cysts of Protozoa

d)

Fungi

e)

Enveloped Viruses

38.

Which of the following is the strongest antimicrobial agents?

a)

Virucidal Liquid

b)

Spermidicial Liquid

c)

Sporidicial Liquid

d)

Fungicidal Liquid

e)

Bacteriostatic Liquid

39.

Which of the following CANNOT be used as antiseptic?

a)

70% alcohol

b)

Crystal Violet

c)

Hydrogen Peroxide

d)

Iodine

e)

Glutaraldehyde

40.

Reduction in bacterial count from 107 to 103 (4 log drop) results in ____________ death

a)

40%

b)

99.99%

c)

100%

d)

90%

e)

30%

41.

Which of the following can disrupt the cell membrane integrity to cause leakage ?

a)

cholesterol

b)

100% alcohol

c)

distilled water

d)

70% alcohol and detergent

e)

detergent only

42.

Moist heat (such as an autoclave) conditions that can sterilize is: 

a)

60 C for 30 min

b)

141 C for 1 second

c)

100 C for 3 Hours

d)

121 C for 15 min

e)

70 C for 15 seconds

43.

Dry heat conditions that can sterilize is:

a)

100 C for 2 Hours

b)

170 C for 60 min

c)

70 C for 2 min

d)

121 C for 15 min

e)

141 C for 10 min

44.

Autoclave

a)

121 C for 15 min

b)

72 C for 15 seconds

c)

140 C for 1 seconds

d)

100 C steam for 30-60 mins

e)

Exposing to a vacuum under freezing conditions

45.

Pasteurization

a)

121 C for 15 min

b)

72 C for 15 seconds

c)

140 C for 1 seconds

d)

100 C steam for 30-60 mins

e)

Exposing to a vacuum under freezing conditions

46.

Ultrapasteurization/UHT

a)

121 C for 15 min

b)

72 C for 15 seconds

c)

140 C for 1 seconds

d)

100 C steam for 30-60 mins

e)

Exposing to a vacuum under freezing conditions

47.

Tyndallization

a)

121 C for 15 min

b)

72 C for 15 seconds

c)

140 C for 1 seconds

d)

100 C steam for 30-60 mins

e)

Exposing to a vacuum under freezing conditions

48.

Lypholization

a)

121 C for 15 min

b)

72 C for 15 seconds

c)

140 C for 1 seconds

d)

100 C steam for 30-60 mins

e)

Exposing to a vacuum under freezing conditions

49.

Pasteurization kills 100% of pathogens from drinks

a)

True

b)

False

50.

Which of the following does NOT cause direct DNA strand breaks?

a)

x rays

b)

cathode rays

c)

gamma rays

d)

ultraviolet light

51.

Which of the following may intentionally be used by scientists to enhance the mutation rates of bacteria?

a)

cathode rays

b)

uv light

c)

x rays

d)

gamma rays

52.

The size of a filter that can filter out bacterial endospore is:

a)

2mm

b)

10um

c)

0.2mm

d)

2000nm

e)

0.2 um (micrometer) or 200 nm

53.

Which of the following is the most effective in killing bacteria?

a)

3% hydrogen peroxide

b)

soap

c)

70% alcohol

d)

100% alcohol

e)

detergent

54.

Which is the following used to be very common in "total toothpaste" and antibacterial soap but now banned ? 

a)

Sodium bi-carbonate

b)

Peroxide

c)

Benzalkonium Chloride

d)

Triclosan

e)

Alcohol

55.

The complex ionic compound found in antibacterial wipes is usually:

a)

 

dimethyl benzyl ammonium chloride (benzalkonium chloride)

b)

eucalyptus oil

c)

20% alcohol

d)

triclosan

56.

Which concentration of alcohol is the most effective in killing bacteria?

a)

21.6%

b)

70%

c)

100%

d)

30%

57.

Which of the following is found is "Peridex" mouthwash?

a)

0.12% chlorhexidine gluconate

b)

70% alcohol

c)

3% hydrogen peroxide

d)

benzalkonium chloride

58.

Which of the following is commonly used for treating burned skins?

a)

70% alcohol

b)

10% bleach

c)

3% hydrogen peroxide

d)

silver sulfadiazaine

e)

4% formalin

59.

Which of the following is commonly added to soft drinks as a preservative?

a)

Chlorhexidine Gluconate

b)

Citric Acid

c)

Ascorbic Acid

d)

Benzalkonium Chloride

e)

Benzoic or sorbic acid

60.

drop from 10^9 to 10^5

a)

90%

b)

99%

c)

99.9%

d)

99.99%

e)

99.999%

61.

drop from 10^7 to 10^2

a)

90%

b)

99%

c)

99.9%

d)

99.99%

e)

99.999%

62.

drop from 10^8 to 10^7

a)

90%

b)

99%

c)

99.9%

d)

99.99%

e)

99.999%

63.

drop from 10^9 to 10^3

a)

90%

b)

99%

c)

99.99%

d)

99.999%

e)

99.9999%

64.

drop from 10^6 to 10^3

a)

90%

b)

99%

c)

99.9%

d)

99.999%

e)

99.9999%

65.

Aerotolerant anaerobes

a)

Lack the metabolic enzyme systems for using oxygen in respiration. Obligate anaerobes also lack the enzymes for processing toxic oxygen and die in its presence

b)

Do not require oxygen for metabolism, but use it when it is present.

c)

oxygen is necessary for metabolism and growth

d)

Do not grow at normal atmospheric concentrations of oxygen but require a small amount of it in metabolism

e)

Do not utilize oxygen but can survive and grow to a limited extent in its presence