WorksheetsQuest #3
Total questions: 20
Worksheet time: 11mins
BUN accounts for how much % of total NPN?
25
10
5
None of the Above
BUN is Whole Blood is approximately how many times higher than that of plasma?
75%
1.75
1.76
0.75
Give the Collective Term of these reagents
Protein Precipitants
Color Reactant
Folin-Wu Protein Precipitants
Nelson-Somogyi Protein Precipitants
The protein precipitant used in DAM method gives a characteristic color of filtrate:
Green
Avocado Green
Chocolate Brown
None of the Above
Give the reason why this glassware is preferred when preparing PFF
Allows accurate dilution to calibration mark
Tapered mouth to facilitate receiving filtrate from the funnel
Allows accurate measurement of liquid
Facilitates mixing by swirling motion
Give the Chemical Name of this compound
2,2-butandione
2,3-butandione
2,2-butanedione
2,3-butanedione
Give the purpose of this reagent
Protein Precipitant
Main Color Reactant
Oxidizing Agent
Color Stabilizer
Destroys the hydroxylamine set free during the reaction
ODD ONE OUT
Test Category: Direct
Endpoint Colorimetric
Yellow diazine measured at 480nm
Color is not photosensitive
What incubator is used for DAM method?
(a)
What is the category of estimation for the BUN "New Method"?
Endpoint Colorimetric
UV Vis Spectrometer
UV Spectrophotometry
UV Kinetic Spectrophotometric
ODD ONE OUT: BUN New Method Reagent
Urease
GLDH
a-ketoglutarate
NAD
End product measured under SPM
Urea
NH3
L-glutamate
NAD
Composition of "S" cuvette
(More than one correct answer)
Equal parts BUN reagent and diluent
10uL standard
20mg/dL urea standard
20uL sample
Incubation time and temperature of the test
(More than one correct answer)
37oC
20 minutes
Room Temperature
5 minutes
Exact wavelength used in the test
340.0 nm
340.4 nm
339.7 nm
341.1 nm
Data points which gives the most consistent data over 60 seconds
(More than one correct answer)
20
30
60
90
BUN New Method: Total Duration of Data Collection
(a)
Compute for the Patient's BUN value:
Initial A of standard = 1.111
Final Absorbance of standard = 1.074
Initial A of unknown = 1.224
Final A of unknown = 1.183
Round off to WHOLE NUMBER. Answer Directly with unit of measurement
(a)
BUN New Method: Convert the patient's value to S.I. units and interpret data
(May select more than one correct answer)
Patient Change in Absorbance = 0.041
Standard Change in Absorbance = 0.037
7.85 mg/dL
7.91 mg/dL
22.16 mg/dL
Above reference Range
Within reference Range
