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Protein analysis

Total questions: 12

Worksheet time: 6mins

Name
Class
Date
1.

What are the general steps in the kjeldahl method?

a)

Distillation - Digestion - Titration

b)

Digestion - Distillation - Titration

c)

Titration - Digestion - Distillation

d)

Titration - Distillation - Digestion

2.

The purpose of adding CuSO4 in the kjeldahl method is ...

a)

for Sample destruction

b)

Catalyst

c)

Increasing the boiling point

d)

Neutralizing the sample

3.

The purpose of adding K2SO4 in kjedahl method is ...

a)

to neutralize the sample

b)

Increasing the boiling point

c)

Catalyst

d)

Sample destruction

4.

During the distillation process, nitrogen is captured by boric acid in the form of ...

a)

Nitrite

b)

Nitrate

c)

Ammonia

d)

Urea

5.

The end point of titration is determined by having .... color.

a)

Green

b)

Dark blue

c)

Red

d)

Pink

6.

In general, how many percent of nitrogen content in the protein?

a)

16%

b)

17%

c)

18%

d)

19%

7.

What is the difference between Dumas method and kjeldahl method?

a)

Dumas method is Time consuming

b)

Dumas methos has affordable equipment

c)

Dumas method use hazard chemicals

d)

Dumas method can analyze many samples

8.

The purpose of adding potassium sodium tartrate in biuret method is ...

a)

to stabilize the cupric ions

b)

To produce peptic bonds

c)

To complex with sodium hydroxide

d)

To absorb color in spectrophotometer

9.

The end point color of biuret method is...

a)

Green

b)

Red

c)

Blue

d)

Purple

10.

The example of primary structure is ...

a)

insulin

b)

nucleic acid

c)

myoglobin

d)

hemoglobin

11.

In Lowry Method, what kind of special reagent is added to the samples?

a)

Tyrosin and Tryptophan residues

b)

Folin Ciocalteau phenol

c)

Biuret reagent

d)

Lowry reagent

12.

What is the most distinguishing element present in proteins?

a)

Nitrogen

b)

Sulfur

c)

Carbon

d)

Hydrogen