WorksheetsMicrobial isolation_SSCG 3673_UTM
Total questions: 11
Worksheet time: 7mins
Identify the plating method.
A: spread plate and B: pour plate
A: pour plate and B: streak plate
A: streak plate and B: spread plate
A: pour plate and B: spread plate
The _____________ technique is best used for isolation of bacterial cultures with distinct colonies
Pour plate
Streak plate
Spread plate
Petri dish, alcohol lamp, test tubes, glass rode and pipette
Point out the plating technique which requires these materials.
Streak plate
spread plate
pour plate
pour and spread plates
_____________promote the growth of certain type of bacteria while inhibiting the growth of the undesired organisms.
nutrient agar
differential media
Eosin Methylene Blue agar
selective media
The __________________ can be used to isolate fecal coliforms.
Eosin Methylene Blue agar
Mannitol Salt agar
Phenylethyl Alcohol agar
MacConkey agar
_______________ media can be used to isolate gram negative bacteria.
EMB agar
MSA
MacConkey agar
PEA agar
Darius has collected some water sample from a lake. He believes that the lake is home to a specific type of halophile from the genus Staphylococcus. Suggest the best isolation medium that Darius could use to confirm his claim.
MacConkey agar
EMB agar
MSA
Chocolate agar
Point out the role/s of these media (TSA, Nutrient agar, peptone water)
as routine culture media
to culture fastidious bacteria
to culture bacteria that do not need extra growth nutrients
to support the growth of a wide variety of bacteria
to inhibit the growth of the undesired organisms
Differentiate pour plate and spread plate methods.
wire loop vs glass rod
broth vs agar
isolation of colonies vs counting number of colonies
subsurface vs surface bacterial growth
How would you describe these figures?
A: Selective medium B: Basal medium
A: Differential medium B: Enrichment medium
A: Basal medium B: Selective medium
A: Enrichment medium B: Selective medium
A: Basal medium B:Differential medium
Identify MOs that can grow in Pour plates.
mesophile
thermophile
obligate anaerobe
microaerophile
