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Microbial isolation_SSCG 3673_UTM

Total questions: 11

Worksheet time: 7mins

Name
Class
Date
1.

Identify the plating method.

a)

A: spread plate and B: pour plate

b)

A: pour plate and B: streak plate

c)

A: streak plate and B: spread plate

d)

A: pour plate and B: spread plate

2.

The _____________ technique is best used for isolation of bacterial cultures with distinct colonies

a)

Pour plate

b)

Streak plate

c)

Spread plate

3.

Petri dish, alcohol lamp, test tubes, glass rode and pipette


Point out the plating technique which requires these materials.

a)

Streak plate

b)

spread plate

c)

pour plate

d)

pour and spread plates

4.

_____________promote the growth of certain type of bacteria while inhibiting the growth of the undesired organisms.

a)

nutrient agar

b)

differential media

c)

Eosin Methylene Blue agar

d)

selective media

5.

The __________________ can be used to isolate fecal coliforms.

a)

Eosin Methylene Blue agar

b)

Mannitol Salt agar

c)

Phenylethyl Alcohol agar

d)

MacConkey agar

6.

_______________ media can be used to isolate gram negative bacteria.

a)

EMB agar

b)

MSA

c)

MacConkey agar

d)

PEA agar

7.

Darius has collected some water sample from a lake. He believes that the lake is home to a specific type of halophile from the genus Staphylococcus. Suggest the best isolation medium that Darius could use to confirm his claim.

a)

MacConkey agar

b)

EMB agar

c)

MSA

d)

Chocolate agar

8.

Point out the role/s of these media (TSA, Nutrient agar, peptone water)

a)

as routine culture media

b)

to culture fastidious bacteria

c)

to culture bacteria that do not need extra growth nutrients

d)

to support the growth of a wide variety of bacteria

e)

to inhibit the growth of the undesired organisms

9.

Differentiate pour plate and spread plate methods.

a)

wire loop vs glass rod

b)

broth vs agar

c)

isolation of colonies vs counting number of colonies

d)

subsurface vs surface bacterial growth

10.

How would you describe these figures?

a)

A: Selective medium B: Basal medium

b)

A: Differential medium B: Enrichment medium

c)

A: Basal medium B: Selective medium

d)

A: Enrichment medium B: Selective medium

e)

A: Basal medium B:Differential medium

11.

Identify MOs that can grow in Pour plates.

a)

mesophile

b)

thermophile

c)

obligate anaerobe

d)

microaerophile