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WorksheetsPlant Biotechnology Unit- 1
Total questions: 15
Worksheet time: 8mins
__________ is / are sterilized using the surface sterilization technique
Media
Culture
Explants
Enzymes
An autoclave operates on a temperature & pressure of _________
115 °C & 10 PSI
121 ° C & 15 PSI
110°C & 25 PSI
125° C & 20 PSI
In On-spot method of Media preparation, PGR's are added
Before Autoclaving
Before adjusting pH
After Autoclaving
In surface sterilization, Tween20 acts as a
Disinfectant
Fumigant
Surfactant
Decontaminant
Dry heat sterilization operates at a temperature range of
100 C - 140 C
120 C - 200 C
110 C - 150 C
160 C - 180 C
Of the following, what is NOT an application of callus culture
Determination of Pact cell volume
Production of transgenic crop plants
Used in suspension cultures
Commercial producion of secondary metabolites
------------ technique is used to sterilize PGR's - Plant growth regulators and Enzymes
Surface sterilization
Dry heat sterilization
Filter sterilization
None of the above
When a single cell or group of cells aggregate in a single media under aeration and agitation, it is known as
Callus culture
Cell suspension culture
Habituation
Acclimatization
Increase in concentration of cytokinins leads to
Flowering
Root proliferation
Shoot proliferation
Root & shoot proliferation
The temperature range that promotes adequate callus formation is
15 C - 21 C
22 C - 28 C
27 C- 32 C
25 C - 30 C
Actively growing callus requires
Increase in auxin concentration
Increase in cytokinin concentration
Balance of auxins and cytokinins concentration
None of the above
----------- is a process of morphogenesis involving formation of organs
Embryogenesis
Organogenesis
Somatic embryogenesis
In _________, tissues undergoes morphogenesis with the formation of callus or suspension cultures
Direct embryogenesis
Indirect organogenesis
Indirect embryogenesis
Direct organogenesis
Low concentration of auxins and high concentration of cytokinins promotes
Callus formation
Root & shoot formation
Root formation
Shoot formation
IEDC- Induced Embryogenic Determined Cells are used in
Direct somatic embryogenesis
Direct organogenesis
Indirect somatic embryogenesis
Parthogenesis
