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WorksheetsClinical Chemistry: Blood Collection and Specimen Considerations
Total questions: 41
Worksheet time: 38mins
Tourniquet application: how many minutes is recommended?
< 1 minute
> 1 minute
If BP cuff is used, inflate to:
40-60 mmHg
120 mmHg
90-180 mmHg
20-40 mmHg
What is the routine antiseptics?
70% isopropyl alcohol
Ethanol assay
Iodophor chlorohexidine
70% alcohol
This may lead to falsely elevated values further enclosure.
70% isopropyl alcohol
Ethanol assay
Iodophor chlorohexidine
70% alcohol
This antiseptic is used for culture?
70% isopropyl alcohol
Ethanol assay
Iodophor chlorohexidine
70% alcohol
This is not recommended by CLSI due to safety and specimen quality issues.
Needle and Syringe
Butterfly/Winged Infusion Set
Evacuated Tube System
This is used for infants and children, hand veins, and difficult-draw situations.
Needle and Syringe
Butterfly/Winged Infusion Set
Evacuated Tube System
This consists of double-headed needle, adapter, color-coded evacuated tube.
Needle and Syringe
Butterfly/Winged Infusion Set
Evacuated Tube System
Standard needle size:
21
20
22
23
Length of standard needle:
+ 15
+ 16
+ 17
+ 18
Recommended angle between needle and vein
15-30 °
45-60 °
90 °
45 °
ALL BUT ONE: removes calcium or calcium chelator
EDTA
Citrate
Oxalate
Heparin
Color code: Lavender, Pink
EDTA
Citrate
Oxalate
Heparin
Color code: Light blue, Black
EDTA
Citrate
Oxalate
Heparin
Color code: Gray
EDTA
Citrate
Oxalate
Heparin
Color code: Green
EDTA
Citrate
Oxalate
Heparin
Inhibits thrombin
EDTA
Citrate
Oxalate
Heparin
ALL but ONE: Color Code RED (Plastic Tube)
Silica
Celite
Diatomite
Thrombin
Color Code: Orange
Silica
Celite
Diatomite
Thrombin
This clot activator additive are recommended for STAT Serum Tests
Silica
Celite
Diatomite
Thrombin
Color code: Silica red / Black, gold
Gel + clot activator
Gel + anticoagulant
This separator tube is not recommended for those with THERAPEUTIC DRUG MONITORING
Gel + clot activator
Gel + anticoagulant
This separator tube's color code is light green/gray
Gel + clot activator
Gel + anticoagulant
It is an antiglycolytic agent which prevents glycolysis and for glucose testing as well as lactate and ethanol assays.
Sodium Fluoride
Thrombin
Sodium Citrate
EDTA
Indications: Newborn screening (blood spots), daily monitoring of DM Patients, POCT
Capillary Puncture
Arterial Puncture
IV Puncture
Indications: arterial blood gas analysis, absence of palpable veins
Capillary Puncture
Arterial Puncture
IV Puncture
Sites of puncture: heel, 3rd or 4th finger, big toe, earlobe
Capillary Puncture
Arterial Puncture
IV Puncture
Sites of puncture: radial, brachial, and femoral
Capillary Puncture
Arterial Puncture
IV Puncture
Capillary Order of Draw:
1. Blood Gases 2. Preparation of Slides 3. EDTA 4. Additive Tubes
1. Preparation of Slides 2. EDTA 3. Blood Gases 4. Additive Tubes
1. Blood Gases 2. Additive Tubes 3. EDTA 4. Preparation of Slides
1. Additive Tubes 2. Preparation of Slides 3. EDTA 4. Blood Gases
Recommended length of lancet blade for heel sticks:
1.75 mm
1.75 cm
17.5 cm
1.75 um
What should be done before using the radial artery? ⭐
(a)
Capillary Order of Draw: To avoid exposure of air
Blood Gases
Preparation of Slides
EDTA
Other Additive Tubes/Serum Tubes
Capillary Order of Draw: To avoid platelet clumping
Blood Gases
Preparation of Slides
EDTA
Other Additive Tubes/Serum Tubes
Amount of Anticoagulant for Arterial Puncture
0.05 mL
1 mL
1.75 mL
2 mL
Phlebotomy Complication: bleeding from venipuncture site and hematoma formation
Vascular
Infections
Anemia (iatrogenic)
Neurologic
Phlebotomy Complication: Second Most Common Complication
Vascular
Infections
Anemia (iatrogenic)
Neurologic
Phlebotomy Complication: Result from blood loss for testing
Vascular
Infections
Anemia (iatrogenic)
Neurologic
Phlebotomy Complication: Particular problem with pediatric patients
Cardiovascular
Dermatologic
Anemia (iatrogenic)
Neurologic
Phlebotomy Complication: Post-phlebotomy seizure or pain
Cardiovascular
Dermatologic
Anemia (iatrogenic)
Neurologic
Phlebotomy Complication: Orthostatic hypotension, syncope, shock, etc.
Cardiovascular
Dermatologic
Anemia (iatrogenic)
Neurologic
Phlebotomy Complication: Allergic reaction to iodine, adhesives, or latex
Cardiovascular
Dermatologic
Anemia (iatrogenic)
Neurologic
