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WorksheetsDECALCIFICATION,DEHYDRATION,CLEARING & IMPREGNATION (QUIZ# 13)
Total questions: 10
Worksheet time: 5mins
DECALCIFICATION can be through the following EXCEPT
Acids
Paraffin
Chelating agents
Electrical ionization
Ion exchange resin
Most commonly used dehydrating agent; recommended for routine procedures; best dehydrating agent; fast-acting, mixes with water, penetrates easily; not poisonous; not expensive
(a)
Process whereby alcohol is removed from the tissue and replaced with a substance that will dissolve the wax which the tissue is to be impregnated.
(a)
Most commonly used colorless clearing agent; clearing time is 15 minutes to 1 hour; used for embedding and mounting procedures
Toluene
Benzene
Xylene
Chloroform
Process whereby the clearing agent is completely removed from the tissue and replaced by a medium that will completely fill all the tissue cavities, thereby giving a firm consistency to the specimen.
(a)
Simplest, most common, and best embedding medium used for routine tissue processing
Celloidin
Gelatin
Paraffin wax
Paraplast
Acid decalcifying agent that decalcifies and softens tissues, nuclear and cytoplasmic staining is good, maceration is avoided due to chromic acid and alcohol; decalcification time is 2-7 days.
Formol
Perenyi's Fluid
HCl
Formic acid
Moderately rapid decalcifying agent, does not require washing out; recommended for teeth and small pieces of bones.
Chromic acid
Citric Acid - Citrate Buffer Solution
Von Ebner's Fluid
Sulfurous Acid
Most ideal and reliable to measure the extent of decalcification; ability to detect even the smallest focus of calcium which appears opaque in plate; not recommended for mercuric-chloride fixed tissues due to its characteristics radio-opacity.
(a)
Used to clear paraffin and celloidin sections during embedding process; clearing time is 2-3 days for paraffin sections and 5-6 days for celloidin sections; recommended for CNS tissues, smooth muscles, skin & cytological studies
Toluene
Cedarwood oil
Aniline oil
Clove oil
