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Molecular markers

Total questions: 14

Worksheet time: 7mins

Name
Class
Date
1.

What is a polymorphism?

a)

The number of genes

b)

A variant in DNA sequence i.e. the number of base repeats

c)

Template DNA

d)

Non of the above

2.

What does RFLP stand for?

a)

Right Fragmented Length of DNA

b)

Restriction Fragment Length Polymorphism

c)

Restriction Enzyme fragment Polymorphism

3.

What does STR stand for?

a)

Single tandem repeat

b)

Single track DNA repeat

c)

Short Track Rest

d)

Short Tandem Repeat

4.

What does VNTR stand for?

a)

Variable Number Tandem Repeat

b)

Restriction enzyme

c)

Variation in Number DNA

d)

Variable number type repeat

5.

What does SNP stand for?

a)

Single stranded DNA

b)

Single nucleus Polymorph

c)

SNIP's

d)

Single Nucleotide Polymorphism

6.

How many Bp is the band is sample A?

a)

1200bp

b)

1000bp

c)

500bp

d)

2--bp

7.

What are the bands in sample B?

a)

1200bp and 800bp

b)

600bp and 200bp

c)

200bp and 800bp

d)

400bp and 900bp

8.

What are the bands in Sample C?

a)

1000bp, 600bp, and 100bp

b)

50bp, 200bp and 400 bp

c)

1200bp, 500bp, and 100bp

d)

600bp, 300bp, and 100bp

9.
The purpose of PCR
a)
cutting DNA
b)
creating RFLPs
c)
amplifying DNA
d)
reversing the charge of DNA
10.
Put the following in order from 1st step to last step.
A. Stain the gel
B. PCR
C. Load the DNA
D. Analyze
E. Add restriction enzymes
F. Electrophorese
a)
B, E, C, F, A, D
b)
D, A, C, B, F, E
c)
F, C, A, E, B, D
d)
B, C, E, A, D, F
11.
How many of these restriction enzymes produce sticky ends?
a)
2
b)
4
c)
5
d)
3
12.
What would be the result of gel electrophoresis if restriction enzymes were not used in the process? 
a)
In theory, there would be only one band produced.
b)
The DNA would move toward the negative electrode instead of the positive electrode.
c)
The DNA would  not be amplified.
d)
Bacteria would be attacked by viruses.
13.
Restriction Fragment Length Polymorphisms
I. are pieces of DNA
II. are all the same size
III. are produced through PCR
IV. are created through the action of restriction enzymes
V. are measured in number of base pairs
a)
I, II, III, IV, and V
b)
I, IV, and V
c)
I, II, and IV
d)
I and IV
14.

The recognition sites for restriction enzymes are :

a)

a).Tautomer

b)

b). Pallindrome

c)

c).Metamere

d)

d).Acronym