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WorksheetsGene Technology
Total questions: 11
Worksheet time: 8mins
Which of the following is a cloning vector?
Gene cloning apparatus
The enzyme that cuts the DNA into fragments
Plasmid which is used to transfer DNA
Bacteriophage that infects bacteria.
What is used in step P?
DNA ligase
EcoRI
Recombinant DNA
Vector
What is used in step Q?
DNA ligase
EcoRI
Recombinant DNA
Vector
To begin the development of the human growth hormone gene, researchers created a cDNA library from cells in the pituitary gland. What does this library contain?
Only the sequence encoding growth hormone
DNA versions of all the mRNAs in the pituitary gland cells
All the coding sequences in the human genome, but no introns
All the coding sequences in the human genome, including introns
The phrases below refer to different stages in the formation of a plasmid-carrying recombinant DNA.
I. Use restriction enzyme
II. Use DNA ligase
III. Remove plasmid from parent bacterium
IV. Introduce plasmid into a new host bacterium
I, II, III, IV
III, I, II, IV
IV, III, II, I
II, III, I, IV
Which of the following is TRUE?
I: d
II: b
III: c
IV: e
V: a
I: a
II: b
III: d
IV: e
V: c
I: d
II: b
III: c
IV: a
V: e
I: a
II: b
III: d
IV: c
V: e
Which of the following diagram representing the cut section of DNA by EcoRI is CORRECT?
A
B
C
D
Name the enzyme P, Q, R & S. Please in such:
P:
Q:
R:
S:
A restriction enzyme breaks down bonds between the
base pairs of a DNA molecule
exons and introns of a DNA molecule
base pairs of a DNA-RNA hybrid molecule
sugar and phosphate components of a DNA molecule
Which of the following is FALSE about plasmid?
found in bacteria
circular in shape
carry few genes
carry foreign genes into animal cells
Write the 7 steps in cloning according to the sequence
