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FIXATION &FIXATIVE LAST QUIZ(QUIZ#15)

Total questions: 10

Worksheet time: 3mins

Name
Class
Date
1.

Solvent used in staining of PARAFFIN SECTIONS

(a)  

2.

Used as a bacterial stain for evaluation and grading of milk, for diagnosis of diphtheria, vital staining of nervous tissue.

a)

Methylene blue

b)

Methylene violet

c)

Toluidine blue

d)

Crystal violet

3.

Nuclear stain used for staining amyloid in frozen sections and platelets in blood.

a)

Methylene blue

b)

Methylene violet

c)

Toluidine blue

d)

Crystal violet

4.

Most commonly used adhesive, easy to make, inexpensive.

(a)  

5.

Mounting medium that is most important; natural resin extracted from Canadian tree, usually dissolve in xylene, for whole mounts and thick sections.

(a)  

6.

Process of sealing the margins of the coverslip to prevent escape and evaporation of the mountant, to immobilize the coverslip, and to prevent sticking of slides upon storage.

(a)  

7.

Fixative that cannot be used in H&E staining since it inhibits hematoxylin.

(a)  

8.

Cell structure that stains BLUE TO BLACK in H&E staining

(a)  

9.

Type of staining method for carbohydrates that stains red or magenta red

a)

De Tomasi

b)

PAS

c)

Itikawa

d)

Oguru

10.

Staining method for MUCIN that is fixed with 10% formalin; Acid mucins/mucopolyssaccharides stains blue and

Nuclei stains red

a)

Toluidine blue

b)

Azure method

c)

Fresh frozen Azure A

d)

Alcian blue technique