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Tutoring 2/3/22

Total questions: 16

Worksheet time: 9mins

Name
Class
Date
1.

What two ends of the amino acid are used to  form the peptide bond?

a)

Carboxyl end of two amino acids

b)

Amino and Hydrogen

c)

Amino and R group

d)

Carboxyl and R group

e)

Amino and Carboxyl

2.

One of the most important protein secondary structures is the alpha helix. This stiff coil is usually 10 or more residues long and forms due to a hydrogen bond between the carbonyl oxygen and the amide nitrogen of amino acids that are ______ residues apart?

a)

5

b)

4

c)

3

d)

2

e)

1

3.

You are trying to deduce the possible tertiary structure of a protein by a reading a series of coding elements (codons) found in its mRNA sequence. You notice the following sequence:  leu-ile-trp-leu. Where would you predict this sequence will be found once the protein is fully folded?

a)

Towards the interior of the protein

b)

Towards the exterior of the protein

c)

Forming the center of a binding site for ATP

d)

Forming the center of a binding site for GTP

4.

Which of these is an amino acid that has a side chain that will function as an acid in a chemical reaction and bear a negative charge at ph=7.2?

a)

Lysine

b)

Arginine

c)

Asparagine

d)

Glutamate

e)

Threonine

5.

Which one of these is an amino acid with an R group, whose pka is between 10-13?

a)

Tryptophan

b)

Asparagine

c)

Isoleucine

d)

Proline

e)

Alanine

6.

What is the main force holding together the secondary structures that form as proteins fold?

a)

Ionic interactions

b)

Interchain disulfide bonds

c)

Hydrophilic and hydrophobic side-chains

d)

Hydrophilic modifications such as adding oligosaccharides

e)

Hydrogen bonds between various parts of the polypeptide backbone

7.

An important structural protein is encoded by a single gene. Cellular analysis indicates that the protein is not modified in a major way following translation, yet there are different versions of the mRNA (long, short & medium-sized) that exist in different tissues such as neurons, kidney & stomach. Which of the following is most likely responsible for the different RNA transcripts?

a)

Genomic recombination

b)

Cytoplasmic recombination

c)

Post-translational modification

d)

Sorting in the Golgi

e)

Alternative RNA Splicing

8.

What RNA is produced by eukaryotic RNA polymerase Ill?

a)

Most of the ribosomal RNA

b)

All of the tRNAs and the 5S rRNA

c)

All the mRNAs

d)

All the processing-RNAs

e)

All of the microRNAs

9.

From this duplex DNA example, which sequence below represents a possible RNA transcript?

5' -GGGCATTTTAAATGC-3'

3' -CCCGTAAAATTTACG-5'

a)

ACGGGCATTTTA

b)

GGGGATTTTAAA

c)

AAATTTTAGGGG

d)

GGGCAUUUUAAA

e)

AAAUUUUACCCC

10.

What DNA-binding protein recognizes a specific bacterial promoter (containing a specific -35 box and -10 box) and acts a detachable subunit to bring the RNA polymerase to the promoter?

a)

The family of Sigma factors

b)

The repressor proteins

c)

Two various activator proteins

d)

The beta subunit of RNA polymerase

e)

The tRNA binding protein that primes the binding of RNA polymerase Il

11.

What is an important structural feature of RNA polymerase Il that is not found in RNA polymerase I or Ill?

a)

It has 5 subunits instead of 4

b)

It possesses the same PCNA collar observed in DNA polymerase delta

c)

It contains a Cap structure

d)

It has a prominent CTD tail

12.

What mediated the Rho-independent termination of transcription in bacteria?

a)

The Rho helicase

b)

The Rho polymerase

c)

The Rho endonuclease

d)

A strong GC-rich RNA hairpin

e)

A small AT-rich RNA hairpin

13.

What RNA is produced by eukaryotic RNA polymerase I?

a)

Most of the ribosomal RNA

b)

All of the tRNAs

c)

All of the mRNAs

d)

All the processing-RNAs

e)

All the microRNAs

14.

Which one of these is an enzyme that is involved in capping a nascent RNA made by RNA Polymerase II?

a)

Adenyl cyclase

b)

Guanylyltransferase

c)

Adenylate transferase

d)

Cryptoform pyrase

e)

Reverse transcriptase

15.

What is the name for the AG site at the 3' end of an intron that is cut by the processing mechanism to release the intron as a lariat structure?

a)

Intron Recognition Site

b)

Exon Recognition Site

c)

Splice Donor Site

d)

Splice Acceptor Site

e)

Branch A Site

16.

What label indicated the 3' end of the template DNA strand?

a)

A

b)

B

c)

C

d)

D

e)

E