Free Printable CHAPTER 2 GENE TECHNOLOGY

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CHAPTER 2 GENE TECHNOLOGY

Total questions: 15

Worksheet time: 12mins

Name

Class

Date

Which of the following is a protein that cleave double stranded DNA molecules at a specific nucleotide sequence?

plasmid

restriction enzyme

ligase

protein expression

Which suspect should be charged with the crime?

Suspect 1

Suspect 2

Suspect 3

None of these suspects

Which of the following is a circular DNA from bacteria that can hold a foreign gene?

plasmid

restriction enzyme

ligase

protein expression

Which of the following is an enzymatic protein that can form phosphodiester bond between sugar and phosphate group between nucleotides?

plasmid

restriction enzyme

ligase

protein expression

Which is true about plasmids?

Plasmids can be cut at specific sequences called restriction sites.

Plasmids contain a promoter sequence that defines where transcription begins.

Plasmids can contain an antibiotic resistance gene.

All of the statements are true about plasmids.

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

rDNA technology

transferrence

transformation

rDNA plasmid

Which of the following is NOT belong to host cell

Able to accept recombinant DNA

Able to accept accept target DNA

Able to maintain structure of recombinat DNA

Able to express the gene of interest

A ____________ is required to transfer genes from one organism to another.

vector

reverse transcriptase

transport molecule

genetic probe

In order to insert a human gene into plasmid, both must

code for the same gene product.

be cut by the same restriction enzyme.

originate from the same type of cell.

have identical sequences.

10.

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells.
IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V) Use ligase to seal plasmid DNA to non-plasmid DNA

I, II, IV, III, V

III, II, IV, V, I

II, III, V, IV, I

III, IV, V, I, II

11.

Which restriction enzyme listed below was isolated from E. coli bacteria?

EcoRI

HindIII

HaeII

SmaI

12.

Which of the following is NOT a tool used in genetic engineering?

plasmid

restriction enzyme

DNA helicase

DNA ligase

13.

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

DNA is negatively charged so attracted to the positive end of the unit

DNA is positively charged to attracted to the negative end of the unit

the agarose gel in negatively charged

the agarose gel is positively charged

14.

In gel electrophoresis, the largest DNA fragment will appear

closest to the starting wells

farthest from the starting wells

three quarters away from the starting wells

it depends on how many fragments there are

15.

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

It allows the strands of DNA to be cut into various lengths for testing

It restricts the number of base pairs that can be tested in a sample

It makes the testing simpler by moving the strands into the gel faster

It charges the DNA strands

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CHAPTER 2 GENE TECHNOLOGY

Total questions: 15

Worksheet time: 12mins

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