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WorksheetsDNA Fingerprinting I
Total questions: 20
Worksheet time: 18mins
Name
Class
Date
1.
The purpose of PCR
a)
cutting DNA
b)
creating RFLPs
c)
amplifying/copy DNA
d)
reversing the charge of DNA
2.
If base-pairing rules state that Adenine always pairs with Thymine, and Cytosine pairs with Guanine, what would be the complementary strand to ACGGT?
a)
ACGGT
b)
TGGCA
c)
TGCCA
d)
ACCGT
3.
What enzyme is used during PCR to pair complementary bases to the template strand?
a)
Thermonuclease
b)
Helicase
c)
DNA Polymerase
d)
RNA Polymerase
4.
How do scientists cut DNA into smaller strands?
a)
restriction enzymes
b)
scissors
c)
ligase
d)
agarose
5.
Based on these results whose blood was found in the blood stain at the crime scene?
a)
Bob
b)
Sue
c)
John
d)
Lisa
6.
The computerized database used to store DNA information is:
a)
AFIS
b)
NIBIN
c)
CODIS
d)
PDQ
7.
Where is DNA found in the body?
a)
Cells nucleus
b)
Heart
c)
Cartilage
d)
Muscle
8.
Which DNA strands move the fastest through the gel when the electricity is turned on?
a)
The long strands
b)
The short strands
c)
The medium strands
d)
They all move the same
9.
Which 2 molecules form the sides (backbone) of the DNA ladder?
a)
deoxyribose sugar and adenine
b)
deoxyribose sugar and a hydrogen bond
c)
deoxyribose sugar and the nucleus
d)
deoxyribose sugar and phosphate
10.
Which man is the father of this baby?
a)
Father 1
b)
Father 2
c)
Father 3
d)
None of them
11.
DNA fingerprinting works because
a)
Most genes are dominant
b)
All organisms contain RNA
c)
The most important genes are different among most people
d)
No two people, except identical twins, have exactly the same DNA
12.
This form of DNA typing uses heat to unzip DNA molecules.
a)
CODIS
b)
RFLP
c)
PCR
13.
Mitochondrial DNA is inherited from:
a)
Just mom
b)
both parents
14.
What enzyme is used during PCR to pair complementary bases to the template strand?
a)
Thermonuclease
b)
Helicase
c)
DNA Polymerase
d)
RNA Polymerase
15.
Biologists need additional copies of a DNA fragment. What tool should they use?
a)
Gel Electrophoresis
b)
PCR
c)
Restriction enzymes
d)
This isn't possible
16.
During PCR, the temperature is increased separate DNA strands, what is this step called?
a)
Extension
b)
Contamination
c)
Annealing
d)
Denaturation
17.
If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?
a)
2
b)
4
c)
8
d)
32
18.
Which region of the DNA sequence below is repeated 3 times in the following sequence:
TGCACGATCATCATCATAGCCCT
a)
TCA
b)
TGC
c)
CAT
d)
TAG
19.
DNA can be degraded or destroyed by ...
a)
moisture
b)
heat
c)
sunlight
d)
all answers
20.
Type of DNA that varies most among individuals and is used to create DNA fingerprints.
a)
non-coding DNA
b)
coding DNA
c)
Deoxyribose
d)
hydrogen bonds
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