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Tutorial Unit 3

Total questions: 15

Worksheet time: 8mins

Name
Class
Date
1.

Bacteria grow through process called?

(a)  

2.

Calculate the No. of cells in population (Nt), given the:

Original No. of cells in population ( No ) = 10

No. of cell division (n) = 15

a)

200, 000

b)

327, 680

c)

500, 000

d)

439, 680

3.

Arrange the phases of bacterial growth CORRECTLY:

I - Death phase

II - Stationary phase

III - Log phase

IV - Lag phase

a)

IV -> III -> II -> I

b)

I -> II -> III -> IV

c)

IV -> II -> III -> I

d)

IV -> III -> I -> II

4.

" Cells are growing fast limited only by growth conditions & genetic potential "

The statement above best describe what stage in bacterial growth curve?

a)

Lag phase

b)

Death phase

c)

Log phase

d)

Stationary phase

5.

Most human pathogen prefer temperature near that of the human body.

They are called:

a)

Psychrophile

b)

Hyperthermophile

c)

Thermophile

d)

Mesophile

6.

Microorganism that only live and grow without oxygen is termed as?

a)

Anaerobe

b)

Aerobe

c)

Facultative Anaerobe

7.

Which of the following organisms requires chocolate agar to grow?

a)

Proteus spp.

b)

Staphylococcus aureus

c)

Neisseria spp.

d)

Escherichia coli

8.

Which of the following media is used to differentiate lactose and non-lactose fermenter?

a)

EMB Agar

b)

Chocolate Agar

c)

Blood Agar

d)

MacConkey Agar

9.

Which organisms can survive in the presence or absence of oxygen?

I - Escherichia coli

II - Staphylococcus aureus

III - Salmonella spp.

IV - Mycobacteria spp.

a)

I, II & III

b)

I & II Only

c)

III Only

d)

I, II, III, IV

10.

Which best describe the following statements?

- Semi solid

- inhibit growth of some organism but allow other to grow

- Example: Mannitol Salt Agar & Eosin Methylene Blue Agar

a)

Enriched Media

b)

Selective Media

c)

Enrichment Media

11.

Green metallic sheen colony as shown in the picture, is the selective characteristics for which organism?

a)

Shigella dysenteriae

b)

Salmonella typhimurium

c)

Escherichia coli

d)

Staphylococcus aureus

12.

The picture best describe the example of which type of culture media?

a)

Simple Media

b)

Transport Media

c)

Anaerobic Media

d)

Enrichment Media

13.

Phototrophs obtain their energy from light?

a)

TRUE

b)

FALSE

14.

Which BEST describe the statements below:

- Autoclave at 121 oC for 15 mins

- Sterile Filtration

- To avoid bacterial contamination

a)

Sterilization of Culture Media

b)

Preparation of Culture Media

c)

Incubation of Culture Media

15.

Which are the tools utilized in techniques used to culture media (Streaking; Swab/ Spreading; Stabbing; Sterilization)?

I - Inoculating loop

II - Inoculating needle

III - Cell spreader

IV - Micro incinerator

a)

IV Only

b)

I, II, III, IV

c)

I & III Only