WorksheetsRecombinant DNA
Total questions: 8
Worksheet time: 8mins
Name
Class
Date
1.
An enzyme that cut DNA molecules at a specific “recognition site” is called
a)
Restriction enzyme
b)
DNA polymerase
c)
RNA polymerase
d)
Taq polymerase
2.
Which of the following is a circular DNA from bacteria that can hold a foreign gene?
a)
plasmid
b)
restriction enzyme
c)
ligase
d)
protein expression
3.
Which of the following is a protein that acts as DNA scissors?
a)
plasmid
b)
restriction enzyme
c)
ligase
d)
protein expression
4.
A restriction enzyme is used to cut both the gene of interest and the plasmid at the restriction sites.
a)
True
b)
False
5.
Which of the following model organisms is often used in rDNA technology to make insulin?
a)
small squid
b)
mouse
c)
fruit fly
d)
E. coli bacteria
6.
The unpaired nucleotides produced by the action of restriction enzymes are referred to as
a)
sticky end.
b)
base sequence.
c)
single strands.
d)
restriction fragments.
7.
In order to insert a human gene into plasmid, both must
a)
code for the same gene product.
b)
be cut by the same restriction enzyme.
c)
originate from the same type of cell.
d)
have identical sequences.
8.
What is the most logical sequence of steps for creating recombinant DNA?
I) Insert the recombinant DNA molecule back into a bacteria cell and let it reproduce.
II) Cut the plasmid DNA and human DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells and locate a human gene of interest.
IV) Sticky ends cause the plasmid DNA to hydrogen-bond to the human DNA fragments.
a)
I, II, IV, III,
b)
III, II, IV, I
c)
II, III, IV, I
d)
III, IV, I, II
100 %
