WorksheetsMICOO1
Total questions: 128
Worksheet time: 1hrs 4mins
The branching filaments of molds and mushrooms
Hyaline
Hyphae
Mycelia
Thallus
Mass of hyphae constructing the thallus
Hyaline
Hyphae
Mycelia
Thallus
Dematiaceous hyphae is also known as the dark colored hyphae, on the other hand , clear hyphae is known as?
Clear Dematiaceous hyphae
Hyaline hyphae
Bleached hyphae
None of the choices
Which of the following statement below is NOT true regarding , Sabouraud dextrose agar (SDA).
Inhibits the growth of many bacteria.
Allows fungal contaminants and pathogenic fungi to grow
Alkaline in pH
Acidic in pH
It is used to demonstrate germ tube production by the yeast Candida albicans
Mycosel agar
Germ tube media
SDA
Birdseen agar
It is used for growth of Cryptococcus neoformans
Mycosel agar
Germ tube media
SDA
Birdseed agar
It is used to recover dermatophytes
Mycosel agar
Germ tube media
SDA
Birdseed agar
Direct exam method used to dissolve nonfungal materials in skin, hair, and nail samples
Calcium hydroxide 10%
Potassium oxide 10%
KOH 30%
None of the choices.
Infection of the scalp Caused by Microsporum and Trichophyton
Tinea capitis
Tinea barbae
Tinea corporis
Tinea cruris
Tinea is a mycotic organism?
TRUE
FALSE
Tinea is the medical term for the infection/s caused by dermatophytic mycotic organism?
TRUE
FALSE
Histoplasma capsulatum does not have any capsule
true
false
Histoplasma capsulatum and Sporothrix schenckii are both dimorphic.
true
falsle
Which of the following organism below is considered to be achlorophyllous
Candida albicans
Aspergillus fumigatus
Histoplasma capsulatum
All of the options.
Which pair below is correctly matched:
FUNGI : APPROX 7um in dm.
FUNGI: EUKARYOTIC
BACTERIA : 4um in dm.
BACTERIA: EUKARYOTIC.
Examine the cell wall below: Given the key elements in this cell wall, this belongs to a:
FUNGI
BACTERIA
VIRUS
PLANT
Displayed below are spores of the bacteria Clostridium tetani (blue background), and of a fungus (black background). Which statements below is false regarding spores.
Spore in fungi is primarily used for reproduction.
Spore in bacteria is mainly dormant.
Spore in bacteria is for heat resistance.
All are TRUE
Perfect fungus characteristic refers to the ability of the organism to reproduce both via sexual and asexual method.
True
False
Both H. capsulatum and C. neoformans can be acquired via bird droppings
true
false
Most systemic mycoses affects primarily the lungs.
true
false
Opportunistic fungi usually have hyaline hyphae.
TRUE
FALSE
This is the temperature of the refrigerator at which all specimens submitted for viral recovery regardless of source should be transported.
5 to -15 C
70 C
-70 C
0-4 C
If Inoculation of the specimen into tissue culture media will exceed 5 days this is the temperature the spcimen should be at:
5 to -15 C
70 C
-70 C
0-4 C
It is a technique that employs single-stranded , complementary nucleic acid probes for detection of human immunodeficiency virus (HIV) in the blood of seronegative individuals
Eastern-Blot technique
Western- Blot technique
Southern-Blot technique
Classify : Picronavirus
Non-enveloped
Enveloped
Classify : Coronovirus
Non-enveloped
Enveloped
Classify : Paramyxovirus
Non-enveloped
Enveloped
Classify : Orthomyxovirus
Non-enveloped
Enveloped
Causative agent of AIDS:
Lentevirus
Orthomyxovirus
Adenovirus
Retrovirus
Causative agent of HEPATITIS B:
Hepadnavirus
Orthomyxovirus
Adenovirus
Picornavirus
Causative agent of HEPATITIS A:
Hepadnavirus
Orthomyxovirus
Adenovirus
Picornavirus
Causative agent of DENGUE:
Flavivirus
Parvovirus
Adenovirus
Picornavirus
Causative agent of MUMPS
Flavivirus
Orthomyxovirus
Adenovirus
Paramyxovirus
Causative agent of Influenza type A
Flavivirus
Orthomyxovirus
Adenovirus
Paramyxovirus
Causative agent of Influenza type B:
Flavivirus
Orthomyxovirus
Adenovirus
Paramyxovirus
It causes a primary disease (chicken pox) and a recurrent disease (shingles)
Papillomavirus
Rotavirus
Varicella zoster virus
Paramyxovirus
The preferred pH range for bacterial growth
ACIDIC
Alkaline
NEUTRAL
The preferred pH range for Mycotic/fungal growth
ACIDIC
ALKALINE
NEUTRAL
The pH range/value which is considered to be Neutral is
pH 8
pH 13
pH 7
pH care
The pH range/value which is considered to be Acidic.
pH 8
pH 13
pH 7
pH 2
The pH range/value which is considered to be Alkaline.
pH 1
pH 13
pH 7
pH 2
Do some fungi produce substances that may kill bacteria?
Yes
No
Maybe
Which of the following organism below is considered to be achlorophyllous
Candida albicans
Aspergillus fumigatus
Histoplasma capsulatum
All of the options
Which statement below is not true regarding fungi.
They exist in nature as saprophytic.
They are resistant to high temperatures.
They grow in large amounts of Sugar.
All are false.
Fungi are said to have no true cell wall but has true roots.
True
False
Fungi are much closely associated to _______ in terms of structure
Plants
Bacteria
Viruses
Which pair below is correctly matched:
FUNGI : APPROX 7um in dm.
FUNGI: EUKARYOTIC
BACTERIA : 4um in dm.
BACTERIA: EUKARYOTIC.
Examine the cell wall: Given the key elements in this cell wall, this belongs to a
FUNGI
BACTERIA
VIRUS
PLANT
The cell wall content of a bacteria is mostly composed of
Chitin
Ergosterol
Peptidogylcan
Displayed below are spores of the bacteria Clostridium tetani (blue background), and of a fungus (black background). Which statements below is false regarding spores
Spore in fungi is primarily used for reproduction.
Spore in bacteria is mainly dormant.
Spore in bacteria is for heat resistance.
All are TRUE
What type of hyphae is responsible for nutrient absorption and stability
Aerial hyphae
Sexual hyphae
Vegetative hyphae
The ability of an organism to grow in more than one form under different environmental conditions
Monomorphic
Anamorphic
Dimorphism
In Lactophenol cotton blue staining, what part of the mixture acts as the mounting agent.
Lactophenol
Cotton blue
Methyl blue
Classify the following condition/disease as to the choices given: Tinea nigra
SUPERFICIAL MYCOSES
CUTANEOUS MYCOSES
SUBCUTANEOUS MYCOSES
Classify the following condition/disease as to the choices given: Mycotic Otitis Externa
SUPERFICIAL MYCOSES
CUTANEOUS MYCOSES
SUBCUTANEOUS MYCOSES
Classify the following condition/disease as to the choices given: Biegel's disease
SUPERFICIAL MYCOSES
CUTANEOUS MYCOSES
SUBCUTANEOUS MYCOSES
Classify the following condition/disease as to the choices given: Presents with patchy "blotchy" desquamating rash.
SUPERFICIAL MYCOSES
CUTANEOUS MYCOSES
SUBCUTANEOUS MYCOSES
1porothrix schenkii is a dimorphic fungus, same as Candida albicans.
True
False
Madura's foot is a condition caused by a fungal specie only.
True
False
JOCK ITCH IS otherwise known :
Tinea pedis
Tinea cruris
Tinea capitis
Trichophyton that has a distinct colony color of Port wine to deep violet.
Trichophyton tonsurans
Trichophyton violaceum
Trichophyton rubrum
HALLMARK: MACROCONIDIA WITH TUBERCULATE-FINGER LIKE PROJECTIONS
C.immitis
B. dermatitidis
P. brasiliensis
H. capsulatum
HALLMARK: INTRACELLULAR YEAST CELLS, MOSTLY RESISTANT TO PHAGOCYTIC ACTION OF MACROPHAGES
C.immitis
B. dermatitidis
P. brasiliensis
H. capsulatum
HALLMARK: COB-WEB LIKE GROWTH OF MYCELIUM.
C.immitis
B. dermatitidis
P. brasiliensis
H. capsulatum
HALLMARK: INTRACELLULAR YEAST CELLS, MOSTLY RESISTANT TO PHAGOCYTIC ACTION OF MACROPHAGES.
C.immitis
B. dermatitidis
P. brasiliensis
H. capsulatum
HALLMARK: MULTINUCLEATED YEAST CELLS WITH MLTIPLE BUDDING.
C.immitis
B. dermatitidis
P. brasiliensis
H. capsulatum
HALLMARK: BROAD BASED BUDDING YEAST CELL, LOLLIPOP APPEARANCE OF CONIDIA.
C.immitis
B. dermatitidis
P. brasiliensis
H. capsulatum
What type of WBC is mostly responsible for the defense against opportunistic fungi (dissimenated form).
T-CELL
B-CELL
PLASMA CELL
BASOPHILS
Spelunker's disease is otherwise known as histoplasmosis. What is Spelunking?
Exploring underwater caves.
Study of exotic bird species.
Exploring caves.
Study of caves and underground structures.
Spherules and Endospores are often seen in the mycotic elements of this fungi.
C. immitis
B. dematitidis
H. capsulatum
Which of the ff. Opportunistic fungi is strictly exogenous in nature:
C. immitis
H. capsulatum
C. albicans
C. neoformans
Microscopic appearance of this fungi is often confused with the amastigote form of L. donovani.
H. nana
H. capsulatum
H. diminuta
C. immitis
Darling's disease is otherwise known as Histoplasmosis. Who is Darling?
I don't know and I don't care.
The name of the patient who first have the infection.
The name of the pathologist who first described the infection.
Darling is not a person, rather a place where it is first discovered.
Histoplasma capsulatum and Sporothrix schenckii are both dimorphic.
true
false
Perfect fungus characteristic refers to the ability of the organism to reproduce both via sexual and asexual method.
True
False
Histoplasma capsulatum does not have any capsule.
true
false
1Both H. capsulatum and C. neoformans can be acquired via bird droppings.
true
false
Most systemic mycoses affects primarily the lungs.
true
false
. A bulldozer operator became ill while working on a new highway in the San Joaquin Valley. He developed chest pain, anorexia, headache and general malaise, and myalgia with fever. Chest X-ray showed pneumonic infiltrate and a single, well-defined nodule in the left lower lobe. His leukocyte count and sedimentation rate were slightly elevated. Although no fungus was seen in direct examination of a sputum specimen, processing included a culture on Sabouraud dextrose agar with chloram- phenicol and cycloheximide. Within 3 days at30°C,this culture produced moist, grayish growth, and white aerial mycelia began to develop (see Color Plate 30B). A lactophenol cotton blue wet mount of this organismisseeninColorPlate31•. Whatis the most likely identification of this fungus?
Asperigillus fumigatus
Blastomyces dermatitidis
Coccidioides immitis
Histoplasma capsulatum
A 38-year-old male from Ohio presented to his physician with a mild influenzalike illness that included headache and malaise. His chest X-ray showed no infiltrates. His past medical history was unremarkable. He had no history of travel but reported recently cleaning the bell tower at his church, which was littered with bird excrement. The most likely agent causing his disease is
Aspergillus fumigatus
Coccidioides immitis
Candida albicans
Histoplasma capsulatum
A 44-year-old gardener pricked herself with a rose thorn. A subcutaneous fungal infection characterized by the develop- ment of necrotic ulcers followed this direct inoculation of fungal spores into the skin. The causative fungus was cultured as a small yeast form at 35°C (see Color Plate 32 •) and as a mould at room temperature with delicate hyphae and conidia. This disease is
Blastomycosis
Chromomycosis
Mycetoma
Sporotrichosis
A yeastlike fungus was isolated from a sputum sample. No hyphae were produced on cornmeal agar with Tween 80. The isolate was negative for nitrate assimilation and positive for inositol assimilation and produced urease at 37°C. These findings are typical of
Candida krusei
Cryptococcus terreus
Cryptococcus neoformans
Trichospown beigelii
A 24-year-old Vietnamese refugee was seen at a clinic in Houston. His chief complaints were weight loss and fever. A complete blood count confirmed he was suffering from anemia as well. Multiple skin lesions were present on his arms, some of them draining pus. Gram stain of the pus revealed what appeared to be yeastlike cells. A culture of the pus grew a green mould at 22°C, which produced a red soluble pigment (see Color Plate 33«). A lactophenol cotton blue wet mount of this organism is seen in Color Plate 34«. The causative agent in this case is
Aspergillus fumigatus
Fusariumsp
Trichoderma sp.
Penicillium marneffei
A section of a lymph node stained with the Gomori silver and hematoxylin and eosin stains is shown in Color Plate 35 •. A lactophenol cotton blue wet mount of a mould that grew from this specimen is shown in Color Plate 36 •. Large, one- celled, smooth to tuberculate macroconi- dia and smooth or echinulate microconidia are typical of mycelial phase growth of
Blastomyces dermatitidis
Coccidioides immitis
Histoplasma capsulatum
Paracoccidioides brasiliensis
Which of the following types of Candida albicans infection is commonly acquired from an exogenous source?
Diaper rash
Neonatal thrush
Perianal infection
Urinary tract infection
In a direct examination of a KOH wet mountofanailspecimen, Epidermophyton floccosum could be detected as
Arthroconidia
Blastoconidia
Macroconidia
Microconidia
The mould phase of the systemic fungus Blastomyces dermatitidis can be confused with
Scedosporium apiospermum
Sporothrix schenckii
Aspergillus sp.
Penicillium notatum
It is usually difficult or impossible to identify a fungal culture before it is mature. However, hyaline, septate hyphae, and a young conidiophore with a foot cell (see Color Plate 37B) and a swollen vesicle are excellent clues to the identification of
Acremonium
Aspergillus
Paecilomyces
Penicillium
Zygomycetes are rapidly growing, airborne saprobes. In clinical specimens they
Are common as normal, human microflora
Are found only as contaminants
May be seen in a dimorphic tissue phase
May be found as a cause of rapidly fatal infection
Trichophyton rubrum and T. mentagrophytes may be differentiated by the
Consistently different appearance of their colonies
Endothrix hair infection produced by T. rubrum
Fluorescence of hairs infected with T. rubrum
In vitro hair penetration by T. mentagrophytes
Broad, coenocytic hyphae found in tissue would be most typical of infection with
Aspergillus
Blastomyces
Microsporum
Rhizopus
A fungus infecting only skin and nails typically produces in culture
Spindle-shaped, hyaline, echinulate macroconidia and microconidia
Cylindrical or club-shaped, smooth, thin-walled macroconidia and microconidia
Many microconidia in clusters or along the hyphae
Large, thin-walled, club-shaped macroconidia without microconidia
The most useful finding for prompt, presumptive identification of C. albicans is its
Failure to assimilate sucrose
"Feathering" on EMB
Production of chlamydospores
Production of germ tubes
Identify the dimorphic fungus that typically has a tissue phase in which the large mother cells have one to a dozen narrow-necked buds and a slowly growing mycelial form with intercalary chlamydoconidia and coiled hyphae
Blastomyces dermatitidis
Coccidioides immitis
Histoplasma capsulatum
Paracoccidioides brasiliensis
Which of the following stains greatly enhances the visibility of fungi by binding to the cell walls, causing the fungi to fluoresce blue-white or apple green?
Rhodamine-auramine
Warthin-Starry
Calcofluor white
Periodic acid-Schiff
The formation of arthroconidia is not an important characteristic in the identification of
Coccidioides
Geotrichum
Trichosporon
Sporothrix
A black pigment produced by colonies growing on bird seed agar is due to
Urease
Phenol oxidase
Sucrose assimilation
Arthroconidia production
Which of the following fungi is not considered an opportunistic pathogen?
Absidia
Aspergillus
Coccidioides
Fusarium
Observation of hyaline or dematiaceous hyphae is an early clue in the identifica- tion of common, airborne fungi. Which of the following genera contains species found as dematiaceous contaminants?
Alternaria
Aspergillus
Fusarium
Penicillium
Which of the following fungi is most likely to be found as a common saprobe and as an agent of keratitis?
Exophiala
Phialophora
Fusarium
Wamgiella
The microscopic identification of Pneumocystis jirovecii is based on the detection of
Arthroconidia in subcutaneous tissue biopsies
Cysts and trophozoites in respiratory specimens
Yeasts in respiratory specimens
Tuberculate macroconidia in lung biopsies
Fungi that undergo asexual reproduction are termed
Imperfect
Perfect
Aseptate
Septate
Hyaline septate hyphae, branched or unbranched conidiophores, and multicelled banana-shaped conidia are characteristic of which of the following
Fusarium
Curvularia
Acremonium
Trichophyton
Which of the following does not correctly describe the yeast Rhodotorula rubra?
It has been isolated from dairy products, soil, and water.
It is the most common fungal cause of diaper rash.
It has been identified as a nosocomial pathogen.
It has been found as a contaminant or commensal in specimens of urine, sputum, and feces.
. A 21-year-old male member of a university track team presents to student health services with a light brown circular lesion on his upper back. The agent most likely responsible for this condition is
Candida albicans
Geotrichum candidum
Malassezia furfur
Fusarium spp.
Which of the following is likely to be found in clinical specimens as normal microflora and as clinically significant isolates?
Aspergillus niger
Paracoccidioides brasiliensis
Penicillium marneffei
Candida albicans
A 4-year-old child's hair is falling out in patches. The hair fluoresces when subjected to the UV light from a Wood's lamp. When the hair is cultured, a white cottony mould grows at 25°C on potato dextrose agar. Microscopically, rare microconidia, septate hyphae, and terminal chlamydospores are seen. Macroconidia are absent. The mould fails to grow on polished rice grains. The causative agent is
Microsporum audouinii
Microsporum gypseum
Trichophyton mentagrophytes
Trichophyton rubrum
In tissues infected with Histoplasma capsulatum
The hyphae usually invade blood vessels
Encapsulated yeast cells are typical
Tuberculate macroconidia are typical
The fungus is usually intracellular
Blastomycosis
Lower Sonoran Life Zone
Mississippi and Ohio River basins
Pigeon roosts
Bat roosts
Coccidiodomycosis
Lower Sonoran Life Zone B
Mississippi and Ohio River basins
Pigeon roosts
Bat roosts
Cryptococcosis
Lower Sonoran Life Zone
Mississippi and Ohio River basins
Pigeon roosts
Bat roosts
Histoplasmosis
Sphagnum moss
Starling roosts
Stagnant fresh water
Colorado RiverValley
Sporotrichosis
Sphagnum moss
Starling roosts
Stagnant fresh water
Colorado RiverValley
The cause of white piedra
Hortaea werneckii
Trichosporon sp.
Piedraia hortae
Fonsecaea compacta
The cause of black piedra
Hortaea werneckii
Trichosporon sp.
Piedraia hortae
Fonsecaea compacta
The cause of tinea nigra
Hortaea werneckii
Trichosporon sp.
Piedraia hortae
Fonsecaea compacta
The cause of tinea versicolor
Aspergillus niger
Malassesia furfur
Microsporum gypseum
Geotrichum Candida
A keratinophilic saprophyte
Aspergillus niger
Malassesiafurfur
Microsporum gypseum
Geotrichum Candida
A cause of otomycosis
Aspergillus niger
Malassesia furfur
Microsporum gypseum
Geotrichum Candida
True hyphae and arthroconidia only
Candida albicans
Geotrichum
Trichosporon
Aspergillus fumigatus
True hyphae, arthroconidia, and blastoconidia
Candida albicans
Geotrichum
Trichosporon
Aspergillusfumigatus
Pseudohyphae, blastoconidia, and chlamydospores
Candida albicans
Geotrichum
Trichosporon
Aspergillusfumigatus
Pseudohyphae and blastospores only
Mucor sp.
Candida tropicalis
Cryptococcus neoformans
Candida albicans
Blastospores only, without hyphae or pseudohyphae
Mucor sp.
Candida tropicalis
Cryptococcus neoformans
Candida albicans
Cryptococcus neoformans
Bone marrow
Cerebrospinal fluid
Chronic draining sinus tract of foot
Chronic interdigital lesion of foot
Histoplasma capsulatum mentagrophytes
Bone marrow
Cerebrospinal fluid
Chronic draining sinus tract of foot
Chronic interdigital lesion of foot
Pseudallescheria boydii
Bone marrow
Cerebrospinal fluid
Chronic draining sinus tract of foot
Chronic interdigital lesion of foot
Trichophyton mentagrophytes
Bone marrow
Cerebrospinal fluid
Chronic draining sinus tract of foot
Chronic interdigital lesion of foot
