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WorksheetsPCR and Gel Electrophoresis
Total questions: 12
Worksheet time: 7mins
What was the purpose of the DNA extraction solution (tube #1) we used before we did PCR?
Break open the cell and nuclear membranes
Put DNA back into cells so we can study it
Increase the Amount of DNA
Decrease the Amount of DNA
To produce billions of copies of a specific GENE
To cut DNA
To remove DNA from the nucleus
What is the function of a primer in PCR?
To identify the particular gene of DNA to be copied by PCR.
To copy DNA.
To remove DNA from the nucleus
How many copies of DNA are made after 1 cycle of PCR? (we did 30 cycles during the last block period).
1
2
1 million
4
In preparation for an electrophoresis procedure, restriction enzymes are added to DNA in order to
convert the DNA into gel
cut the DNA into fragments
change the color of the DNA
produce longer sections of DNA
What is the force that separates the DNA fragments by size during gel electrophroesis?
Magnetic force
Gravitational force
Electrical force
Jiu Jitsu
Match the step of PCR with what it accomplishes.
Denaturation
Separates the 2 strands of DNA
Annealing
Primers attach to the DNA
Extension
Copy of the gene is created
Taste buds are...
Enzymes in saliva
Receptor proteins
Hormones released by salivary glands
In a classroom PTC lab, a student can taste PTC. Which of the following can you conclude?
PTC tasting is the dominant allele and follows incomplete dominance.
The student must be TT ONLY
The student must be Tt ONLY
The student could be TT or Tt
The student is tt
