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WorksheetsPglo Lab
Total questions: 9
Worksheet time: 5mins
What is genetic transformation?
A prokaryotic cell turning into a eukaryotic cell
Foreign DNA being inserted into a plasmid
A host organism taking in foreign DNA
A gene being mutated
What is the purpose of the Luria broth (LB) when performing genetic transformation?
Provides resistance to antibiotics
Provides nutrients needed for bacteria growth
Stabilizes the plasmid being given to the host organism
Sterilizes the instruments
A "-ampR" cell
has the ampR gene in it's plasmid and is resistant to ampicillin
does not have the ampR gene in it's plasmid and is resistant to ampicillin
has the ampR gene in it's plasmid and is not resistant to ampicillin
does not have the ampR gene in it's plasmid and is not resistant to ampicillin
Which is the correct sequence for the process of genetic transformation?
bacterial cells transferred to cold CaCl2 , plasma with foreign DNA added bacteria , cells heat shocked at 42oC , cells spread on agar petri dish , bacteria given time to grow on petri dish
bacterial cells transferred to cold CaCl2 , cells heat shocked at 42oC , plasma with foreign DNA added bacteria , bacteria given time to grow on petri dish , cells spread on agar petri dish
plasma with foreign DNA added bacteria , bacterial cells transferred to cold CaCl2 , cells spread on agar petri dish , bacteria given time to grow on petri dish , cells heat shocked at 42oC
Which one of these agar plates contains bacteria most like the original bacterial culture
+pGLO LB/amp
+pGLO LB/amp/ara
-pGLO LB/amp
-pGLO LB
Which one of these agar plates would there be no bacteria growth?
+pGLO LB/amp
+pGLO LB/amp/ara
-pGLO LB/amp
-pGLO LB
The plasmids scientists use in a lab are MOST COMMONLY found in
plants
animals
archaea
bacteria
Arabinose is used in the plates to
isolate and select for only the bacteria that have been successfully transformed
give bacteria energy to grow
act as a trigger to turn on the expression of the gene of interest
give the bacteria a new trait (such as fluorescence or insulin production)
