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Waters HPLC Troubleshooting and Quality Parts Seminar

Total questions: 10

Worksheet time: 3mins

Name
Class
Date
1.

Which of the following are the benefits of Waters Quality Parts?

a)

Waters Quality Parts are tested on Waters system – designed specifically for LC optimal performance

b)

Critical clean process during manufacturing to ensure parts are handled properly – greatly reduces chances of contamination

c)

Peace of mind that the valuable LC investment is protected – system warranty is not invalidated by installing third party parts

d)

All of the above

2.

Which statement is correct?

a)

Waters recommends a Performance Maintenance once every two years

b)

All the critical wear-and-tear components are replaced during the Performance Maintenance regardless of the parts’ conditions

c)

User self-performed maintenance is sufficient to ensure system performance

d)

Quality of the mobile phase will not affect the quality of chromatogram

3.

Which is not correct about the troubleshooting workflow?

a)

Having a system benchmark helps me to have a good chromatogram to refer to when there is a suspected LC problem

b)

I am encouraged to use my own sample to setup as system benchmark

c)

Failure fingerprint should have information on how the chromatogram fails and what causes it to fail

d)

Comparing between failure fingerprint helps me to remove any inconsistent failure causes

4.

What is/are the cause(s) of peak shape problem?

a)

Poor fitting

b)

Column void

c)

Plugged column

d)

Silanol activity from column

e)

All of the Above

5.

QCRM from Waters can be used to? (Choose all the answers that applies)

a)

Benchmark system performance

b)

Perform routine PQ checks

c)

Diagnose/troubleshoot system issues

d)

Tell which parts to change of the system

6.

Under the current USP 621 guideline, which is the chromatographic parameter that can be adjusted without revalidation?

a)

Gradient method

b)

Detector wavelength

c)

Different L listing column

d)

Column length and particle size for isocratic method

7.

Part-part manakah yang kemungkinan besar bermasalah Jika RSD RT tidak masuk (>2%) Pada system HPLC anda?

a)

Solvent Filter, Inlet Filter, Checkvalve dan Seal-seal Plunger

b)

Solvent filter, Needle, needle Seal dan sample Syringe

c)

Inlet Filter, Check Valve, Injector valve dan flowcell detector

d)

Needle Seal, needle, Sample Syringe dan Injector valve

8.

Part-part manakah yang kemungkinan besar bermasalah Jika RSD Area tidak masuk (>2%) Pada system HPLC anda?

a)

Solvent Filter, Inlet Filter, Checkvalve dan Seal-seal Plunger

b)

Solvent filter, Needle, needle Seal dan sample Syringe

c)

Inlet Filter, Check Valve, Injector valve dan flowcell detector

d)

Needle Seals, needle and Sample Syringe

9.

Hal-hal berikut ini dapat menyebabkan peak yang dihasilkan mempunyai nilai tailing yang tinggi, Kecuali?

a)

Interaksi sampel dengan stationary phase dalam kolom

b)

Pemasangan tubing pada kolom tidak sempurna

c)

Kolom terdegradasi/rusak

d)

Sampel menguap

10.

Manakah penyebab yang paling sering kolom mengalami blocking/ mampet?

a)

Algae / Bakteri

b)

Kesalahan memilih filter

c)

Fasa gerak buffer

d)

Semua jawaban benar