WorksheetsHISTOPATH Tissue Processing
Total questions: 15
Worksheet time: 11mins
Method of staining where the tissue is first overstained to obliterate the cellular details, and the excess stain is removed or decolorized from unwanted parts of the tissue, until the desired intensity of color is obtained
(a)
What is the usual length of a plane-concave knife?
(a)
It facilitates complete removal of transition solvents and prolongs the life of wax by reducing solvent contamination
(a)
The following are characteristic of a good mounting medium except: Choose all correct answer
It should be freely immiscible with xylene and toluene.
It should set when crystallizing, cracking or shrinking
It should not leach out any stain or affect staining
It should not dry to a non-stick consistency and harden relatively quickly
Which of the following is a correct match?
Canada Balsam Refractive Index: 1.524
Synthetic
DPX
Refractive Index: 1.542
Synthetic
CLARITE Refractive Index: 1.544
Synthetic
XAM
Refractive Index: 1.52
Natural
It is very useful in cytology particularly for cytology, particularly spin preparations of proteinaceous or bloody material
APES
XAM
AMES
SKL
Used as a counterstain in gram staining
(a)
Given the formula:
Ferric ammonium sulfate 2.5 gm Distilled water 100 ml
Hematoxylin 1.5 gm 95% ethyl alcohol 10 ml Distilled water 90 ml
Mayer's Hematoxylin
Weigert's Hematoxylin Solution
Shumachers Hematoxylin Solution
Heidenhain's Hematoxylin
It is used for demonstrating mitochondria during intravital staining.
(a)
This is where tissue is placed into a precisely arranged position in a mold containing a medium which is the allowed to solidify.
(a)
How many changes of wax are required d to remove the clearing agent and properly impregnate the specimen in automatic processing?
(a)
TRUE OR FALSE: clearing agent and diluted ethanols should be changed at least once a week
(a)
Infiltrating and Embedding medium should be molten between __ and __
30°C and 50°C
45°C and 70°C
20°C and 40°C
30°C and 60°C
It consists of two L-shaped strips of heavy brass or metal arranged on a flat metal plate and which can be moved to adjust the size of the mold to the size of the specimen
(a)
What is the main reason why cells are stained?
(a)
