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Antibody Screening

Total questions: 14

Worksheet time: 9mins

Name
Class
Date
1.

The purpose of LISS (Low Ionic Strength Solution) as enhancer is to:

A- Identify nuisance antibodies present

B- Stabilize and prepare blood samples

C- Prevent possible hemolysis of cells

D- Promote antigen-antibody reactivity

a)

A

Identify nuisance antibodies present

b)

B

Stabilize and prepare blood samples

c)

C

Prevent possible hemolysis of cells

d)

D

Promote antigen-antibody reactivity

2.

The source of antigens used for antibody detection consists of ______

a)

AB cells

b)

O cells

c)

H cells

d)

MNS cells

3.

Used to demonstrate the presence of autoantibodies

a)

AHG

b)

blood grouping test

c)

Autocontrol

d)

indirect antibody test

4.

Indirect Coombs is

a)

Tests for antibodies in the serum

b)

detect in vivo coating of RBCs

c)

Can be used to help diagnose HDN

d)

Complemenary structure of antibody molecule and epitope

5.

When one or more of the ____ D antigen are missing, a weak D phenotype may be expressed

a)

capsid

b)

N-DRC

c)

envelope

d)

epitopes

6.

the phenotypic expression of common red cell antigens for each vial has been established and provided in the form of an (a)  

7.

Antibodies that demonstrate dosage

a)

ABH, Rh, Lewis, Kidd, P, I

b)

Rh, Duffy, Kidd, MNS, Lutheran

c)

MN, Duffy, Lutheran, Ch, Rg

d)

Lea, I/i

8.

If the antibody screen shows reaction at 37C, is it most likely to be an IgG or IgM antibody?

(a)  

9.

Reverse grouping is to

a)

known reagent antisera to detect ABO antigens on the patient's red blood cells

b)

known reagent screening cells to detect clinically significant antibodies in the patient's serum or plasma

c)

known reagent red blood cells to detect ABO antibodies in the patient's serum or plasma.

d)

known reagent antisera to phenotype donor red blood cells for clinically significant antigens.

10.

A former patient from O&G had an anti-E two years ago, but her antibody panel is now negative. Since she now needs blood for cesarean surgery, what should the blood bank do?

a)

Give random compatible units.

b)

Get autologous blood from relatives.

c)

Crossmatch E-negative units.

d)

Give group O negative whole blood.

11.

What is the maximum interval during which a recipient sample may be used for compatibility testing?

a)

3 days

b)

48 hours

c)

2 months

d)

1 day

12.

You perform a ABO/Rh test on a 54 years old female patient and you obtain the following results.

Anti-A - 4+

Anti-B - 4+

Anti-D - 4+

D control - negative

A1 cells - 2+

B cells - 0

What could be the Type/Rh of this patient?

a)

A1B positive

b)

invalid

c)

A2B positive

d)

O positive

13.

Bombay phenotype

a)

hh

b)

HH

c)

O'hh

d)

ABOH

14.

Anti-H antisera is prepared from

a)

pig mucosa

b)

Ulex europeaus

c)

Vicia graminae

d)

Iberis amara